罗默碱对梨锈病菌抑制机理的初步研究  

Preliminary Studies on the Antifungal Mechanism of Roemerine Against Gymnosporangium haraeanum Syd.

作　　者：刘小红[1] 邓业成[1] 唐煌[2] 骆海玉[1] 宁蕾[1] 秦卉[1] 

机构地区：[1]广西师范大学生命科学学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004 [2]广西师范大学,药用资源化学与药物分子工程教育部重点实验室,广西桂林541004

出　　处：《北方园艺》2011年第12期122-124,共3页Northern Horticulture

基　　金：珍稀濒危动植物生态与环境保护省部共建教育部重点实验室研究基金资助项目(桂科能1001Z014);广西自然科学基金资助项目(桂科自0991097)

摘　　要：以生长速率法测定罗默碱对梨锈病菌菌丝生长的抑制作用,以倍比稀释法测定罗默碱最小抑菌浓度(MIC)和最小杀菌浓度(MFC),以外渗电导法测出罗默碱对梨锈病菌菌丝体细胞膜渗透性的影响,并且测定了罗默碱对菌体可溶性蛋白含量的影响,探讨罗默碱对梨锈病菌的抑制活性及抑制机理。结果表明:罗默碱对梨锈病菌有较强的抑制活性,且浓度越高,抑制作用越强,其MIC为0.25 g/L,MFC为0.5 g/L;用0.2 g/L的罗默碱处理梨锈病菌菌丝体,结果显示其细胞膜的相对渗透率为53.49%,高出对照21.82%;用0.2 g/L的罗默碱处理梨锈病菌菌丝体,其蛋白质含量为2.5 mg/g,对蛋白质合成的抑制率为60.06%。说明罗默碱对梨锈病菌具有较强的抗菌作用;可影响梨锈病菌菌丝体细胞膜的渗透性和可溶性蛋白的含量。Fungal inhibitory activity of roemerine against G.haraeanum Syd was determined using a growth rate method.And doubling dilution method was used to determined the minimal inhibitory concentration（MIC） and minimal fugicide concentration（MFC）,leakage conductivity method using roemerine mycelia of G.haraeanum Syd cell membrane permeability,and determined roemerine on soluble protein content of cell.To explore the antifungal activity and potential mechanism of roemerine against Gymnosporangium haraeanum Syd.The vesults showed that,Roemerine displayed strong antifungal activity to G.haraeanum Syd and the activity was significantly increased when the applied concentration was higher,the MIC value of 0.25 g/L and MFC value of 0.5 g/L.The results showed that roemerine at a concentration of 0.2 g/L was the relative membrane permeability 53.49%,21.82% higher than the control;G.haraeanum Syd protein content of roemerine at a concentration of 0.2 g/L was 2.5 mg/g,protein synthesis inhibition rate of 60.06%.Roemerine had a strong antifungal activity and exertd a potent impact on the mycelial cell membrane permeability and soluble protein content of G.haraeanum Syd.

关 键 词：罗默碱 梨锈病菌 抑制活性 抑制机理 

分 类 号：S436.612.29[农业科学—农业昆虫与害虫防治]