温度对侧耳菌处理秸秆饲料营养价值及纤维分解酶活性的影响  

Studies on optimal temperature and time to treat corn stalk by Pleurotus ostreatus

作　　者：刘敏[1] 李杰[1] 刘坦[1] 荆义[1] 

机构地区：[1]东北农业大学动物科技学院,黑龙江哈尔滨150030

出　　处：《草业学报》2011年第5期87-92,共6页Acta Prataculturae Sinica

基　　金：东北农业大学博士启动基金项目(2010RCB3)资助

摘　　要：试验旨在研究温度对侧耳菌处理玉米秸秆饲料营养价值及纤维分解酶活性的影响。在16,20和24℃温度条件下,采用侧耳菌处理玉米秸秆饲料,测定处理秸秆纤维素、半纤维和木质素含量、纤维素和木质素分解酶活性及秸秆养分瘤胃消失率。结果表明,在3个温度条件下,处理秸秆的纤维素、半纤维素和木质素含量均随时间的延长而降低。16℃组3种纤维含量降低速度(P<0.05)、酶活性(P<0.01)及干物质瘤胃消失率(DMD48)(P<0.05)显著低于20和24℃组,而20℃组与24℃组以上指标没有显著差异,这2组秸秆DMD48随着时间延长而增加,21d后趋于稳定。结果显示温度显著影响侧耳菌处理秸秆饲料的纤维含量和营养价值,16℃处理秸秆显著低于20～24℃,而20℃与24℃处理秸秆饲料的纤维含量和营养价值差异不显著。The contents in the rumen of fibrous matters,enzymatic activity,and nutrient degradability of corn stalk treated by Pleurotus ostreatus was studied at 16,20,and 24℃ to find the optimal range of treatment temperature and time to achieve a greatest nutritional value of corn stalk.P.ostreatus fermentation reduced the contents of cellulose,hemicellulose,and lignin in stalks treated at the three temperatures.Following treatment of corn straw with P.ostreatus,fibrous matter was degraded slower（P0.05） and the activity of fibrolysis enzymes（P0.01） and dry matter degradability were reduced（P0.05） in the rumen at 16℃ compared with treatment at 20 and 24℃ but were not significantly different between the temperatures.Dry matter degradability in the rumen of stalks treated at 20 and 24℃ increased with treatment time until 21 days of fermentation,and then remained constant.The optimal conditions to treat corn straw with P.ostreatus to achieve a greater nutritional value for ruminant animals is between 20 to 24℃ for 21 to 30 days or for 30 days at a minimum of 16℃.

关 键 词：温度 侧耳菌 玉米秸秆 营养价值 纤维分解酶活性 

分 类 号：S816[农业科学—饲料科学]