菊苣航天诱变材料RAPD分析及高产品系筛选  

RAPD analysis of Cichorium intybu by space mutagenesis and high-yield strains' selection

作　　者：韩永芬[1,2] 卢欣石[1] 舒健虹 付薇 陆瑞霞 覃涛英 

机构地区：[1]北京林业大学林学院草业科学,北京100083 [2]贵州省草业研究所,贵州贵阳550006

出　　处：《草业学报》2011年第5期133-141,共9页Acta Prataculturae Sinica

基　　金：贵州省创新能力建设项目(黔科合院所创能[2009]4013);贵州省农业攻关项目(黔科合NY字[2009]3083)资助

摘　　要：菊苣是一种从国外引进的优良牧草品种,产量高、适口性好,现已在生产中广泛应用。该研究以菊苣航天诱变材料和普那、将军2个主推菊苣品种共29份材料为研究对象进行RAPD分析和产量研究。从100个RAPD随机引物中筛选出的多态性强、重复性好且稳定性高的引物15个,15个引物共扩增出78条带,其中多态性带为63条,比例为87.77%。聚类结果表明,所选菊苣材料之间的遗传多样性较低,相似性系数在0.69～0.80,其中材料PA186单成一支,与其他菊苣材料相似性较低。材料之间的聚类关系与表型特征呈不完全的相关性。从菊苣牧草生产性能看,产量高于目前主推品种黔引普那菊苣(9.5kg/m2)和将军(9.7kg/m2)的品系有12个。Cichorium intybu is a excellent forage with high yield and good palatability,which was introduced from abroad.It has been used in production widely.The genetic relationship and production performance of 29 C.intybu were studied in this research by using RAPD method.The result showed that 15 primers selected from 100 random primers were characteristic of high polymorphism,repeatability and stability.78 DNA fragments were amplified,including 63 polymorphic bands.The average percentage of polymorphic bands was 87.77%,cluster analysis showed that genetic similarity coefficient of C.intybus was ranging from 0.69 to 0.80,there was a lower genetic diversity among germplasm resources of C.intybus tested.PA-186 clustered alone.The fingdings indicated an incomplete correlation among the dendrogram and phenotypic characteristics.According to production performance,the yield of twelve new lines were higher than Puna chicory（9.5 kg/m2）and Jiangjun chicory（9.7 kg/m2）.

关 键 词：菊苣 航天诱变 RAPD 高产品系 

分 类 号：S548[农业科学—作物学]