HPLC-UV法检测土壤中的3-硝基苯并蒽酮  

Determination of 3-nitrobenzanthrone in Surface Soil by HPLC-UV

作　　者：郭金芝[1] 丰硕[1] 金东日[1] 

机构地区：[1]延边大学理学院化学系,吉林延吉133002

出　　处：《延边大学学报（自然科学版）》2011年第3期249-253,共5页Journal of Yanbian University（Natural Science Edition）

摘　　要：建立了HPLC-UV法检测土壤中多环芳烃类物质3-硝基苯并蒽酮的方法.采用on UltimateXB-C18(5μm,150mm×4.6mm i.d.)柱,流动相为甲醇∶水=7∶3(v/v),流速为0.5mL/min,柱温为30℃,检测波长为391nm.在此色谱条件下,3-硝基苯并蒽酮在0.2～40μg/mL范围内具有良好的线性关系,最低浓度检测限为78ng/mL(S/N=3).利用超声波萃法对土壤中的3-硝基苯并蒽酮进行了萃取,并用HPLC-UV法测出了其含量.A HPLC-UV method for the determination of 3-nitrobenzanthrone（3-NBA） in surface soil was developed.HPLC analysis was performed on Ultimate XB-C18（5μm,150mm×4.6mm i.d.） with MeOH-water as the mobile phase.The flow rate kept at 0.5mL/min,the column temperature was set to 30℃ and the detection wavelength was 391nm.The linear range is 0.2-40μg/mL for 3-NBA.The detection limit is 7.8ng/mL（S/N=3）.Using ultrasonic extraction,3-NBA in soil samples were extracted,and tested the content of 3-NBA by HPLC-UV.

关 键 词：3-硝基苯并蒽酮 HPLC-UV 土壤样品 多环芳烃 

分 类 号：X502[环境科学与工程—环境工程]