荷叶总生物碱清除氧自由基作用的研究  

Study on Scavenging Oxygen Free Radicals Capabilities of Total Alkaloids from Lotus Leaves

作　　者：肖桂青[1] 田云[1] 卢向阳[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《化学与生物工程》2011年第10期35-38,共4页Chemistry & Bioengineering

基　　金：湖南省教育厅优秀青年基金资助项目(05B026);湖南农业大学后备人才创新团队建设基金资助项目(04TD04)

摘　　要：应用微弱化学发光法考察荷叶总生物碱对氧自由基的清除作用,并与荷叶水提液、荷叶醇提液及维生素C进行比较。结果表明,荷叶总生物碱对3种氧自由基O2-.、.OH、H2O2的IC50分别为305μg.mL-1、50μg.mL-1、3700μg.mL-1,其清除.OH的能力最强,显示了较强的抗氧化活性;荷叶水提液、荷叶醇提液仅对O2-.、.OH具有一定的清除能力,其中对.OH的清除能力强于维生素C。Weak chemiluminescence method was used to research the scavenging abilities on O-2·,·OH and H2O2 of total alkaloids from lotus leaves,and compared with those of aqueous extract of lotus leaves,alcohol extract of lotus leaves and vitamin C.The results showed that the IC50 of scavenging O-2·,·OH,H2O2 for total alkaloids from lotus leaves were 305 μg·mL-1,50 μg·mL-1,3700 μg·mL-1,respectively.The total alkaloids from lotus leaves had the stronger antioxidant activity with the strongest scavenging ability on ·OH.Aqueous extract and alcohol extract of lotus leaves had a certain scavenging abilities on O-2·,·OH,and their scavenging abilities on·OH were stronger than that of vitamin C.

关 键 词：荷叶总生物碱 氧自由基 抗氧化活性 

分 类 号：TQ464.4[化学工程—制药化工] R282.71[医药卫生—中药学]