三甲基硅酯化/GC/MS法分析荞麦籽中的脂肪酸  

Analysis of Fatty Acid Composition of Buckwheat Seeds by Trimethylsilyl-Esterifying/GC/MS

作　　者：古元梓[1] 杨杰 魏永生[1] 郑敏燕[1] 

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000

出　　处：《化学与生物工程》2011年第10期88-90,94,共4页Chemistry & Bioengineering

基　　金：陕西省教育厅自然科学基金资助项目(2010JK902);咸阳师范学院科研基金资助项目(09XSYK208)

摘　　要：以无水乙醚为溶剂,采用索氏抽提法提取荞麦籽中的粗脂肪,先分别采用三甲基硅酯化法和氢氧化钾/甲醇碱催化甲酯化法对粗脂肪进行衍生化处理,再通过气相色谱/质谱联用技术分析脂肪酸的组成。结果表明,通过三甲基硅酯化法处理,粗脂肪提取率达4.96%,共鉴定出18种脂肪酸成分,主要为亚油酸(34.69%)、油酸(31.08%)、棕榈酸(17.19%)、硬脂酸(2.83%)、亚麻酸(2.27%)和二十三烷酸(2.27%)。与氢氧化钾/甲醇碱催化甲酯化法相比,三甲基硅酯化法是一种更全面的分析荞麦籽脂肪酸组成的衍生化方法。Rude fat from buckwheat seeds was extracted by Soxhler extraction method with ethyl ether as solvent.After being derived by trimethylsilyl-esterifying with N,O-bis（trimethylsilyl） acetamide and methyl-esterifying catalyzed by KOH/MeOH,the fatty acid was analyzed by GC/MS.The results showed the extraction rate of fatty acid was 4.96% and 18 kinds of fatty acids were obtained through trimethylsilyl-esterifying treatment.The main components were linoleic acid（34.69%）,oleic acid（31.08%）,palmitic acid（17.19%）,stearic acid（2.83%）,linolenic acid（2.27%）,ricosanoic acid（2.27%）.Comparing with the methyl-esterifying catalyzed by KOH/MeOH,the trimethylsilyl-esterifying could more thoroughly analyze the fatty acid composition of buckwheat seeds.

关 键 词：荞麦 脂肪酸 三甲基硅酯化法 甲酯化法 气相色谱/质谱法 

分 类 号：TQ646.4[化学工程—精细化工] O657.63[理学—分析化学]