慢性乙型肝炎患者乙型肝炎病毒耐药基因型研究  

Research on the drug-resistant genotypes of hepatitis B virus in patients with chronic hepatitis B

作　　者：张书楠[1] 余文辉[1] 周大桥[2] 张春雷[1] 冯玉丽[1] 贺劲松[2] 周小梅[1] 李伟雄[1] 陈雪红[1] 林卓玲[1] 

机构地区：[1]广东省深圳市中医院检验科,518033 [2]广东省深圳市中医院肝病科/国家级肝病重点专科,518033

出　　处：《国际检验医学杂志》2011年第16期1797-1799,共3页International Journal of Laboratory Medicine

基　　金：深圳市科技计划项目(201003194)

摘　　要：目的研究乙型肝炎病毒(HBV)基因型与耐药病毒株产生的相关性。方法收集85例接受拉米夫定治疗1年以上的慢性乙型肝炎(CHB)患者为研究对象。采集血样经离心分离血浆,进行HBVDNA定量检测,再进行基因测序和基因分型。结果基因测序和基因型分析结果为A型4例(4.7%),B型28例(32.9%),C型37例(43.5%),D型11例(12.9%),B/C混合型5例(5.9%)。其中发生YMDD耐药突变的基因型突变率分别是A型1例(25.0%),B型5例(17.9%),C型16例(43.2%),D型2例(18.2%),B/C混合型1例(20.0%)。结论 HBV基因型C型发生YMDD耐药突变的频率较高,HBV基因型与YM-DD突变可能存在相关性。Objective To explore the correlation between hepatitis B virus （HBV） genotypes and the resistance to lamivudine（LAM）.Methods 85 cases of patients with chronic hepatitis B（CHB）,who had received LAM therapy for more than one year,were recruited. HBV DNA in blood samples of the 85 patients were quantitatively detected,sequenced and genotype were analyzed.Results Sequencing and genotyping revealed that 4（4.7%） cases were with genotype A,28（32.9%） with genotype B,37（43.5%） with genotype C,11（12.9%） with genotype D and 5（5.9%） with mixed genotype B/C. The frequency of mutations in YMDD motif in patients with genotype A was 25.0%,with genotype B was 17.9%,with genotype C was 43.2%,with genotype D was 18.2% and with mixed genotype B/C was 20.0%,respectively.Conclusion The frequency of mutations in YMDD motif in HBV genotype C could be higher than that in the other genotypes. It might be deduced that there could be a correlation between HBV genotypes and mutations in YMDD motif of HBV DNA.

关 键 词：肝炎病毒 乙型 基因 突变 

分 类 号：R512.62[医药卫生—内科学]