呼肠孤病毒对乳腺癌的治疗作用  

Efficacy of Reovirus Against Breast Cancer

作　　者：朱敬之[1] 陈珏[2] 董胜祥[3] 严惟力[4] 吴志勇[1] 

机构地区：[1]上海交通大学医学院附属仁济医院普外科,上海200127 [2]上海交通大学医学院,上海200127 [3]上海交通大学医学院附属苏州九龙医院老年科,江苏苏州215021 [4]上海交通大学医学院附属仁济医院核医学科,上海200127

出　　处：《标记免疫分析与临床》2011年第5期314-320,共7页Labeled Immunoassays and Clinical Medicine

摘　　要：为研究溶瘤呼肠孤病毒对乳腺癌的治疗作用,采用NOD/SCID鼠建立人乳腺癌组织块异种原位移植瘤模型,腹腔注射呼肠孤病毒,3天后通过HE和TUNEL观察乳腺癌的治疗效果。结果显示:没有雌激素辅助的免疫缺陷鼠,肿瘤无法长出。在移植人乳腺癌标本的同时给予免疫鼠雌激素,则移植成功率大为提高,可以达到29.6%(P<0.01)。移植瘤模型的雌激素受体(ER)均为阳性。应用病毒后,乳腺癌组织细胞裂解消失,组织疏松,凋亡倍增。结论:NOD/SCID鼠移植瘤模型具有与原发癌一致的生物学特性。呼肠孤病毒对乳腺癌有治疗作用。To investigate the role of oncolytic reovirus in breast cancer,a tumor xenograft model of NOD/SCID mice was established using a biopsy sample of a primary infiltrating ductal carcinoma obtained from a breast cancer patient.The result of HE and TUNEL was analyzed after injecting the reovirus peritoneally for 3 days.The results showed that estrogen supplementation was required to establish appropriate human breast cancer xenograft model of NOD/SCID mice.29.6% of these transplanted tumors grew with supplementation of Estrogen.Otherwise none grew（P,0.01）.ER of the xenograft model was positive.After treatment with reovirus for 3 days,breast cancer cells were disrupted and disappeared which induced tissue looseness.The rate of apoptosis increased double than before.The biological characteristics of tumor xenograft model confirm with the primary breast cancer.The oncolytic reovirus can kill breast cancer in short time.

关 键 词：乳腺癌 溶瘤呼肠孤病毒 NOD/SCID鼠 

分 类 号：R737.9[医药卫生—肿瘤]