某市801例宫颈人乳头瘤病毒感染快速检测及其意义  

Rapid detection and analysis of human papilloma virus infection in 801 cases of female patients in certain city

作　　者：罗福康[1] 余娟春[1] 李蒙[1] 陈浩[1] 刘畅[1] 龙川江[1] 

机构地区：[1]第三军医大学新桥医院检验科,重庆400037

出　　处：《国际检验医学杂志》2011年第17期1934-1936,共3页International Journal of Laboratory Medicine

摘　　要：目的研究重庆市妇女宫颈人乳头瘤状病毒(HPV)亚型感染现状、分布特点及高危因素。方法采用快速导流杂交基因芯片分型技术对2009年6月至2010年12月于本院妇产科门诊就诊的801例患者进行宫颈HPV基因型检测,并以HE染色对标本进行病理染色分析。结果 801例受检者中HPV感染总阳性率为41.82%(335/801),检测21种HPV亚型。HPV感染率在30～40岁年龄段达高峰,50岁后明显下降。在感染类型中,单一型感染率为26.2%(210/801),双重感染率为9.5%(76/801),多重感染率为6.1%(49/801)。各种宫颈病变与HPV感染率之间的关系为随宫颈疾病病变程度的加重,HPV总感染率及高危型HPV感染率均显著上升,差异有统计学意义(相关系数为0.999,P<0.01)。结论重庆市HPV感染分布以HPV-31、6、52、16和11亚型为主。HPV感染与年龄密切相关,年龄是HPV感染的一个重要的相关因素。随着宫颈疾病病变程度的加重,HPV总感染率及高危型HPV感染率均显著上升。Objective To investigate the current situation,distribution character and high risk factors of human papilloma virus（HPV） subtype infection in women of Chongqing City.Methods 801 cases of female outpatients in Xinqiao Hospital were enrolled and detected for HPV genotypes by DNA flow-through hybridization gene chip technique,and HE staining was performed for pathological diagnosis,between July 2009 to December 2010.Results The total positive rate of HPV infection was 41.82%（335/801）,and 21 subtypes were found.The positive rate of HPV infection in 30 to 40 years old group was the highest,and then decreased since 50 years old.The single,double and multiple infection rates were 26.2%（210/801）,9.5%（76/801） and 6.1%（49/810） respectively.With the aggravation of cervix diseases,the positive rates of total HPV infection and high risk subtype HPV infection increased（r=0.999,P0.01）.Conclusion The major HPV subtypes infection in Chongqing were HPV-31,6,52,16 and 11.Age could be one of the risk factors of HPV infection.The cervix pathological changes could have a close relation with HPV infection and high risk HPV subtypes infection.

关 键 词：宫颈肿瘤 人乳头瘤状病毒 导流杂交 

分 类 号：R737.33[医药卫生—肿瘤]