榄香烯抑制Raf/MEK/ERK信号通路诱导人源胶质瘤U87细胞凋亡  

Induction of elemene on apoptosis of U87 glioblastoma cells by inhibiting Raf/MEK/ERK pathway

作　　者：赵永顺[1] 吴春明[2] 董斌[2] 位振清[2] 徐英辉[2] 

机构地区：[1]大连医科大学,大连116044 [2]大连医科大学附属第一医院神经外科,大连116011

出　　处：《实用药物与临床》2011年第5期365-367,共3页Practical Pharmacy and Clinical Remedies

摘　　要：目的探讨莪术提取物榄香烯体外诱导胶质瘤细胞凋亡的机制。方法采用流式细胞术、West-ern印迹等方法,分别检测不同浓度榄香烯对人源U87胶质瘤细胞的凋亡诱导及其对U87细胞Raf-1、ERK、癌基因Bcl-2蛋白质表达的影响。结果榄香烯对人源U87胶质瘤细胞具有明显的凋亡诱导作用,该增殖抑制效应呈时间依赖性。榄香烯可明显下调U87细胞的磷酸化Raf-1、ERK、Bcl-2表达。结论体外榄香烯对胶质瘤细胞具有明显的凋亡诱导作用(呈时间依赖性),抑制Raf/MEK/ERK信号通路,从而下调其下游信号癌基因Bcl-2的表达,最终启动凋亡程序可能是榄香烯诱导U87细胞凋亡的机制。Objective To study the mechanism by which elemene induces the apoptosis of human U87 glioblastoma cells in vitro.Methods Flow cytometry,Western blotting analysis w as used to test the effect of elemene on apoptosis of U87 glioblastoma cells,expression of Raf-1,ERK,Bcl-2 protein at different concentrations respectively.Results Elemene induced apoptosis of U87 glioblastoma cells in a dose-dependent manner.Elemene downregulated the expression of phosphorylated Raf-1,ERK and Bcl-2.Conclusion Elemene induced apoptosis of U87 glioblastoma cells in vitro.Inhibiting Raf / MEK / ERK pathw ay and dow nregulation of Bcl-2 might be the mechanism of anti-proliferation effect of elemene.

关 键 词：榄香烯 胶质瘤 RAF-1 ERK 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]