机械牵张力作用下小鼠成骨细胞骨形成蛋白2的浓度变化  

Effects of mechanical strain on bone morphogenetic protein 2 concentration of osteoblasts

作　　者：卢红飞[1] 麦志辉[1] 艾虹[1] 徐晔[1] 王伟[1] 陈奇[1] 

机构地区：[1]中山大学附属第三医院口腔科,广州510630

出　　处：《中华口腔医学研究杂志（电子版）》2011年第5期24-27,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省科技计划(2008B030301063)

摘　　要：目的研究不同大小机械牵张力对成骨细胞骨形成蛋白2(BMP-2)浓度的影响。方法通过对小鼠成骨样细胞分别施加0%、6%、12%和18%形变率的机械牵张力72h后,用酶联免疫吸附剂测定(ELISA)法检测BMP-2浓度的变化。其结果经SPSS13.0统计软件进行LSD检验。结果各实验组间以及与对照组间BMP-2浓度差别有统计学意义(P<0.01);12%拉伸形变组BMP-2浓度量最大。结论一定大小的机械牵张力可以促进成骨细胞BMP-2蛋白的表达,从而影响正畸治疗中的骨改建。Objectives To investigate the effect of different magnitudes of mechanical strain on bone morphogenetic protein 2 （BMP-2） protein expression in osteoblasts.Methods Rats osteoblasts were exposed to different mechanical strains.The elongation in Group A （control group） was 0%,while Group B was 6%,Group C was 12%,and Group D was 18%,respectively.Ezyme-linked immunosorbnent assay （ELISA） was used to detect the expression of BMP-2 protein.Data were analyzed by SPSS 13.0 system using LSD analysis.Results There were significant differences among three experimental groups and the control group （P0.01）,and Group C expressed the highest level of BMP-2 protein.Conclusions Mechanical strain can promote the expression of BMP-2 protein,which might affect bone remodeling in orthodontic treatment.

关 键 词：机械牵张力 成骨细胞 骨形成蛋白2 

分 类 号：R783.5[医药卫生—口腔医学]