大麻素受体1和2在肝纤维化模型C57小鼠肝组织中的表达  

Hepatic expression of cannabinoid receptor 1 and 2 in C57 mice with experimental cirrhosis

作　　者：叶立红[1] 王翀奎[2] 刘云燕[1] 杨莉[1] 赵召霞[1] 姜惠卿[3] 戴二黑[1] 

机构地区：[1]河北省石家庄市第五医院检验科,050021 [2]河北医科大学西山校区教务处 [3]河北医科大学第二医院消化科

出　　处：《中国综合临床》2011年第10期1015-1018,共4页Clinical Medicine of China

基　　金：中国肝炎防治基金会王宝恩肝纤维化研究基金项目（20080013）;河北省卫生厅指令性课题（08190）

摘　　要：目的观察大麻素受体1（CB1）和受体2（CB2）在肝纤维化模型C57小鼠肝组织表达及意义。方法30只C57小鼠被随机分为3组，即正常对照组、模型对照组和模型秋水仙碱组，每组10只。采用四氯化碳（CCl4）腹腔注射制造小鼠肝纤维化模型，通过免疫组织化学染色方法检测肝组织中CB1和CB2的表达，同时进行肝组织炎症（G）评分和纤维化（S）评分。结果模型对照组、模型秋水仙碱组及正常对照组肝组织G评分及S评分比较，差异有统计学意义（F=125．41，P=0．00；F=99．18，P=0．00），且模型对照组肝组织G评分及s评分均显著高于模型秋水仙碱组，差异均有统计学意义（P均〈0．01）。模型对照组、模型秋水仙碱组肝组织及正常对照组CB1和CB2评分比较，差异有统计学意义（F=29．27，P=0．00；F=36．99，P=0．00），且模型对照组肝组织CB1和CB2评分均显著高于模型秋水仙碱组，差异具有统计学意义（P〈0．05或P〈0．01）。模型对照组和模型秋水仙碱组中CB1、CB2、G和S评分四者之间均具有相关性（P均〈0．05），且随着G、S评分的不断增加，CB1和CB2评分逐渐增加。结论CB1和CB2在肝纤维化模型C57小鼠肝组织中表达均明显增强，与肝组织炎症和肝纤维化程度有显著相关性。Objective To investigate hepatic expressions and significances of cannabinoid receptor 1 （CB1） and cannabinoid receptor 2（CB2） in C57 mice with experimental cirrhosis. Methods Thirty C57 mice were randomly divided into three groups, i. e. normal control group, model control group and model colchicine group. Hepatic fibrosis model was prepared by intraperitoneal injection of carbon tetrachloride. The expressions of CB1 and CB2 in liver tissue of mice were observed by immunohistochemistry. The scores of inflammation grade （G） and fibrosis stage （S） were simultaneously performed. Results The scores of G and S in model control group and model colchicine group were significantly higher than those in normal control group（F = 125.41 ,P = 0. 00;F = 99. 18, P = 0. 00）. The scores of G and S in model control group were significantly higher than those in model colchicine group（P 〈 0. 01 ）. The scores of CB1 and CB2 expressions in model control group and model colchicine group were significantly higher than those in normal control group （ F = 29. 27, P = 0. 00 ; F = 36. 99, P =0. 00）. The scores of CB1 and CB2 in model control group were significantly higher than those in model colchicine group（ P 〈13. 05 or P 〈 0. 01 ）. There were significant relationships among scores of CB1, CB2, G and S in model control group and model colchicine group（Ps 〈0. 05）. As the scores of G and S became higher,the expressions of CB1 and CB2 gradually became more intensive. Conclusion The hepatic expressions of CB1 and CB2 in C57 mice with experimental cirrhosis increased significantly and have significant relationship with the grades of liver tissue inflammation and fibrosis.

关 键 词：大麻素受体1 大麻素受体2 四氯化碳 肝纤维化 肝损伤 动物模型 

分 类 号：R575.2[医药卫生—消化系统]