E-钙粘素、γ-连环素及基质金属蛋白酶-9的表达与原发性肝癌侵袭转移的关系  

Relationship of expression of E-cad,γ-cat and MMP-9 with invasion and metastasis in human hepatocellular carcinoma

作　　者：李登欣[1] 李石 

机构地区：[1]潍坊市人民医院消化内科,山东潍坊261041 [2]威海卫人民医院消化内科

出　　处：《胃肠病学和肝病学杂志》2011年第9期852-854,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究细胞粘附分子E-钙粘素(E-cadherin,E-cad)、γ-连环素(γ-catenin,γ-cat)及基质金属蛋白酶-9(matrix metallo-proteinase9,MMP-9)与原发性肝癌侵袭转移的关系。方法采用免疫组织化学方法,对经过石蜡包埋的40例原发性肝细胞癌组织和40例癌旁正常肝组织标本进行检测,并结合相关临床病理指标进行半定量分析。结果 E-cad、γ-cat及MMP-9在肝癌中的阳性表达率分别为30.0%(12/40)、32.5%(13/40)、77.5%(31/40),在癌旁正常肝组织中的阳性表达率分别为100%(40/40)、100%(40/40)、17.5%(7/40)。E-cad、γ-cat在肝癌组织中不同程度的表达减弱或缺失,在癌旁正常肝组织中全部正常表达,MMP-9在肝癌组织呈现高表达,在癌旁正常肝组织中呈现低表达或不表达,三者的表达随肝癌的侵袭转移而有显著性差异(P<0.05),且E-cad、γ-cat在肝癌组织中的表达都与MMP-9的表达水平呈负相关(r=-0.562,P<0.05;r=-0.649,P<0.05)。结论 E-cad、γ-cat及MMP-9参与肝癌的侵袭转移过程,在原发性肝癌恶性进展方面起着重要作用。Objective To investigate the correlation of expression of E-Cad,γ-cat and MMP-9 with invasion and metastasis in human hepatocellular carcinoma（HCC）.Methods The expression of E-cad,γ-cat and MMP-9 were detected immunohistochemically in 40 cases of HCC and 40 adjacent normal hepatic tissues.The results were densitometrically semiquantitated combined with related clinical pathological indicators.Results The positive expression rates of E-cad,γ-cat and MMP-9 were 30.0%（12/40）,32.5%（13/40）,77.5%（31/40）in HCC respectively,but 100%（40/40）,100%（40/40）,17.5%（7/40）in the adjacent normal hepatic tissues respectively.The expression of E-cad and γ-cat reduced or were negative in HCC,but E-cad and γ-cat expressed normally in all adjacent normal hepatic tissues.MMP-9 showed high expression in HCC,low or no expression in the adjacent normal hepatic tissues.The expression of E-cad,γ-cat and MMP-9 had significant difference（P〈0.05）with invasion and metastasis of HCC.Furhermore,the expression of E-cad,γ-cat respectively were related with the expression of MMP-9（r=-0.562,P〈0.05;r=-0.649,P〈0.05）.Conclusion The expression of E-cad,γ-cat and MMP-9 involves in the process of invasion and metastasis,play important roles in malignancy progress of HCC.

关 键 词：E-钙粘素 Γ-连环素 基质金属蛋白酶-9 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]