STAT3 shRNA增强胰腺癌细胞Panc-1对吉西他滨化疗敏感性  

STAT3 shRAN enhances chemosensitivity of pancreatic adencarcinoma cell line to gemcitabine hydrochloride

作　　者：张勇[1] 李德春[2] 戴赛民[1] 朱东明[2] 郭子健[1] 周鹏[1] 周俊晶[1] 

机构地区：[1]苏州大学附属无锡第四人民医院肝胆外科,无锡市214062 [2]苏州大学附属第一医院普通外科

出　　处：《江苏医药》2011年第19期2255-2258,共4页Jiangsu Medical Journal

摘　　要：目的观察shRNA下调胰腺癌Panc-1细胞STAT3 mRNA表达后对吉西他滨化疗敏感性的影响。方法构建靶向STAT3 mRNA的shRAN和阴性质粒,分别转染Panc-1细胞(A组和B组);另将非转染的正常Panc-1细胞分为C组和D组。A,B,C三组细胞与吉西他滨共孵育。RT-PCR和Western blot检测Panc-1中STAT3 mRNA和蛋白的表达;MTT法检测Panc-1细胞增殖能力;流式细胞术检测细胞周期。结果 A组STAT3 mRNA水平下降了61.9%,STAT3蛋白水平下降了85.7%(P<0.01),细胞增殖能力低于B、C组(P<0.01)。与B、C组比较,A组G1期细胞比率增加(P<0.05),细胞存活率降低(P<0.01)。结论靶向STAT3 mRAN的shRNA可特异性降低Panc-1细胞中STAT3 mRNA及其蛋白的表达,抑制Panc-1细胞增殖,使其阻滞于G1期,从而增强吉西他滨对G1期细胞的细胞毒效应,增加吉西他滨的化疗敏感性。Objective To investigate the effect of STAT3 shRNA on the expression of signal transducer and activator of transcription（STAT3）,cell proliferation and chemotherapeutic sensitivity of pancreatic adencarcinoma cell-1（Panc-1） to gemcitabine hydrochloride.Methods Two kinds of shRNA plasmids were constructed and transfected Panc-1 cells by LipofectamineTM 2000,one of which targeted STAT3 mRNA（group A）,and the other did not target as negative control（group B）.The normal Panc-1 cells were taken as the control groups of C and D.The Panc-1 cells in groups of A,B and C were cocultured with gemcitabine hydrochloride.The expressions of STAT3 mRNA and its protein were detected with RT-PCR and Western blot.The distribution of cell cycles was examined by flow cytometry and the ability of cell proliferation was analyzed by MTT assay.Results The expression level of STAT3 mRNA decreased by 61.9% and that of STAT3 protein reduced by 85.7% in group A,which were more than those in groups of B and C（P0.01）.So did the ability of cell proliferation（P0.01）.Compared to groups of B and C,the percentage of Panc-1 cells in G1 stage was greater（P0.05） and the cell survival rate was lower（P0.01）.Conclusion STAT3-targeted ShRNA can specifically down-regulate the expressions of STAT3 mRNA and its protein and suppress Panc-1 cells growth with longer stay in G1 stage,which may enhance chemotherapeutic sensitivity of Panc-1 to gemcitabine hydrochloride through intensifying cellular toxic efficacy of the drug.

关 键 词：RNA干扰 信号转导和转录激活因子3 胰腺癌 吉西他滨 化疗 

分 类 号：R735[医药卫生—肿瘤]