宫颈癌组织中Hpa与DEK基因表达的相关性研究  

Research on the relationship between the expression of DEK and Hpa in uterine cervical carcinogenisis

作　　者：刘岿然[1] 杨雨[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医师杂志》2011年第9期1165-1168,共4页Journal of Chinese Physician

基　　金：辽宁省高等学校科研项目计划（2008862）

摘　　要：目的研究DEK与Hpa基因在宫颈癌组织中的表达及二者的相关性。方法采用免疫组织化学方法对宫颈癌石蜡标本45例、宫颈上皮内瘤样病变（CIN）45例、正常宫颈组织10例进行Hpa及DEK基因检测。结果（1）DEK在宫颈癌组织、CIN、正常宫颈组织中阳性表达率分别为88．9％、24．4％、10．0％。宫颈癌组织中DEK阳性表达率高于CIN组织和正常组织（X2=38．05，P〈0．01；）（2=5．77，P〈0．05）；Hpa基因在宫颈癌组织、CIN及正常宫颈组织中的阳性表达率分别为75．6％、24．4％和10．0％，宫颈癌组织中Hpa阳性表达率高于CIN组织和正常组织（x2=31．69，12．94，P〈0．01）。（2）在宫颈癌组织中DEK基因表达与Hpa基因表达呈正相关（r=0．617，P=0．001）。结论宫颈癌组织及CIN组织中DEK、Hpa的表达共同影响CIN的发展及宫颈癌的发生和发展。Objective To investigate the relationship between the expression of DEK and Hpa infection in uterine cervical carcinogenisis. Methods Formalin-fixed, paraffin-embedded samples including 45 cases of squamous cell carcinomas,25 cases of cervical intraepithelial neoplasia CINIII, and 20 cases of CINI-II, 10 cases of normal cervical tissues were studied using polymer detection system for immunohistochemical technique to examine the expression of DEK and Hpa. Results The positive ratios of DEK in cancer tissues, normal cervical intraepithelial lesions, normal cervical tissues positive rates were 88.9%, 24.4%, 10. 0% ,respectively. The positive ratio of expressions of DEK in cervical carcinomas was significantly higher than CIN and normal cervical tissues （X2 =38.054, P 〈0. 01 ;X2 =5. 774, P 〈0.05）. The positive ratios of expressions of Hpa in normal cervical tissues, CIN and cervical cancers were 75.6%, 24.4% and 10. 0% ,respectively. The positive ratio of expressions of Hpa in cervical carcinomas was significantly higher than CIN and normal cervical tissues （ X2 = 31.69, P 〈 0.01 ;X2 = 12. 94, P 〈 0.01 ）. The expression of DEK and Hpa in cervical lesions was highly related in the cervical cancer tissues（ r = 0. 617, P = 0. 001 ）. Conclusions DEK and Hpa possibly played an important role in the development of cervical cancer. Cervical intraepithelial lesions and cervical carcinoma might be associated with changes in the expression of DEK and Hpa, and interact together to influence the development of CIN and cervical cancer.

关 键 词：宫颈肿瘤/代谢 肝素裂合酶/代谢 重组融合蛋白质类/代谢 

分 类 号：R737.33[医药卫生—肿瘤]