白细胞介素-1受体拮抗剂抑制兔颞下颌关节骨关节炎软骨破坏的研究  

Effect of interleukin-1 receptor antagonist on cartilage repair in rabbit temporomandibular joint with osteoarthritis

作　　者：陈瞰[1] 满城[1] 胡静[2] 张碧[1] 祝颂松[2] 

机构地区：[1]四川大学华西口腔医学院口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西口腔医学院口腔颌面外科,成都610041

出　　处：《实用口腔医学杂志》2011年第5期589-592,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:30700951);教育部博士点新教师基金(编号:JS20071226506549)

摘　　要：目的:研究关节腔内注射白细胞介素-1受体拮抗剂对颞下颌关节骨关节炎关节软骨修复的影响。方法:取新西兰大白兔24只,用关节盘部分切除致使穿孔法建立双侧颞下颌关节骨关节炎模型。4周后,在每只动物右侧(实验侧)颞下颌关节腔内一次性注射重组人白细胞介素-1受体拮抗剂50μg,左侧(对照侧)关节腔内注射等量安慰剂。注射后12、24周分别处死12只动物,取双侧颞下颌关节标本进行组织学及RT-PCR检测。结果:双侧关节软骨均呈现出不同程度的骨关节炎病损,对照侧病损更严重。实验侧组织学分数明显高于对照侧(P<0.05)。注射后12和24周时实验侧II型胶原及聚集蛋白聚糖mRNA表达高于对照侧(P<0.05);12周时实验侧关节软骨聚集蛋白聚糖酶mRNA表达低于对照侧(P<0.05),24周时两侧无明显差异(P>0.05)。肿瘤坏死因子-αmRNA表达两侧无明显差异(P>0.05)。结论:颞下颌关节腔内注射IL-Ra能缓解骨关节炎导致的软骨病损,可望成为治疗颞下颌关节骨关节炎的一种新方法。Objective：To investigate the effect of intra-articular administration of interleukin-1 receptor antagonist（IL-1Ra） on cartilage repair in rabbit temporomandibular joint（TMJ） with osteoarthritis（OA）.Methods：Bilateral disc perforation was performed in 24 rabbits to induce osteoarthritic TMJs.4 weeks after surgery,50 μg recombinant human IL-1Ra was injected into each right joint and the contralateral joint received vehicle injection as the control.Twelve animals were sacrificed at 12 weeks and 24 weeks after the injection respectively.Histology and RT-PCR examination were conducted.Results：The vehicle-treated joint had typical OA-related cartilage degradation,whereas the histological score of the IL-1Ra-treated joints were less severe than that in the controls（P0.05）.At 12 and 24 weeks a higher expression of aggrecan and collagen-Ⅱ were observed in the treated joints than that in the controls.Lower expression of aggrecanase was found in IL-1Ra treated TMJs at 12 week after injection.However,no difference in the expression of aggrecanase and TNF-α was found between two groups at 24 weeks.Conclusion：Intra-articular injection of IL-1Ra into TMJ may be a alternative treatment of cartilage degeneration in OA.

关 键 词：IL-1受体拮抗剂 颞下颌关节 骨关节炎 兔 

分 类 号：R782.6[医药卫生—口腔医学]