LRP16与ART-27的相互作用的鉴定  

Identification of ART-27 interaction with LRP16

作　　者：杨洁[1,2] 韩为东[1] 赵亚力[1] 伍志强[1] 

机构地区：[1]解放军总医院基础医学研究所分子生物室,北京100853 [2]解放军总医院老年心血管病研究所,北京100853

出　　处：《现代肿瘤医学》2011年第10期1915-1918,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30870969;81072019)

摘　　要：目的:鉴定LRP16与ART-27的相互作用,推测LRP16基因在肿瘤发生、发展中涉及到的信号途径。方法:酵母双杂交系统筛选出ART-27可与LRP16相互作用,采用酵母双杂交回复验证、免疫共沉淀及GST pull-down等体内、体外方法对二者之间的相互作用进行验证。结果:LRP16与ART-27在体内、体外均可形成复合物。结论:ART-27是LRP16的特异性结合蛋白,二者的相互作用为进一步研究LRP16在胚胎发育及肿瘤发生、发展中的功能及信号传导途径奠定了基础。Objective：To identify the interaction between human LRP16 and ART-27,and to speculate the signal pathway of LRP16 on the development and progression of cancer.Methods： The interaction of ART-27 with LRP16 was found by yeast two-hybrid screening.And this interaction was tested by co-immunoprecipitation analysis in vivo and GST pull-down assay in vitro.Results： LRP16 could bind ART-27 in vivo and in vitro.Conclusion： ART-27 might be a binding partner of LRP16.The interaction between these two proteins may establish a foundation for further studying the function of LRP16 on embryonic development and cancer.

关 键 词：LRP16 ART-27 蛋白相互作用 免疫共沉淀 GST pull-down 

分 类 号：R730[医药卫生—肿瘤]