非小细胞肺癌p53、FHIT、K-RAS基因突变与吸烟相关性Meta分析  

p53,FHIT,K-RAS gene mutations are associated with smoking in non-small cell lung cancer by Meta-analysis

作　　者：马列[1] 赵明静[1] 王群[1] 李秀林[1] 王笑歌[1] 

机构地区：[1]中国医科大学附属第四医院呼吸科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2011年第10期1982-1987,共6页Journal of Modern Oncology

基　　金：国家科技支撑计划课题(编号:2007BAI24B04);中华医学会基金项目(编号:070103200400;50033;8020680146);辽宁省教育厅(编号:05L469 2009A760);辽宁省科技厅自然基金项目(编号:20092097)

摘　　要：目的:从循证医学的角度对非小细胞肺癌患者p53、FHIT、K-RAS基因突变异常表达与吸烟相关性进行Meta分析。方法:检索中国学术期刊网全文数据库(CNKI)、中国科技期刊数据库(维普资讯网)、美国国立图书馆PubMed数据库,美国临床肿瘤学会(ASCO)论文集,辅以手工检索;检索时间段为1990年-2010年。检索出p53、FHIT、K-RAS基因突变与吸烟关系研究的文献197篇,最终符合纳入标准的文献26篇。对纳入文献进行方法学质量评价,采用Meta分析专用软件Review Manager 5.0进行统计分析。结果:共纳入26个研究,分别对有相同统计内容且可以合并统计的p53、FHIT、K-RAS基因突变与吸烟关系合并OR值并计算95%CI。分别为:吸烟人群p53基因突变率较高,表现为低表达,[OR=3.50,95%CI(2.45-5.00),总体效应检验Z=6.88,P<0.0001];吸烟人群K-RAS基因突变率明显偏高,表现为高表达,[OR=4.50,95%CI(3.00-6.75),总体效应检验Z=7.26,P<0.0001];吸烟人群FHIT基因突变率较高,表现为低表达,[OR=2.99,95%CI(1.01-8.88),总体效应检验Z=1.98,P=0.005]。结论:吸烟与p53、FHIT、K-RAS基因突变率呈正相关,其中p53、FHIT基因高表达为保护性因素,K-RAS基因高表达为危险因素。Objective：To perfrome a Meta analysis on the relation of p53,FHIT,K-RAS gene mutations and abnormal expression in non-small cell lung cancer patients with smoking.Methods： Chinese Academic Journals Online（CNKI）,China Science and Technology Periodical Database（VIP Information Network）,the U.S.National Library of PubMed database and the U.S.Society of Clinical Oncology（ASCO） proceedings were searched from 1990 to 2010.A systematic review identified 197 prospective studies concerning p53,FHIT,K-RAS genes and smoking,from which 26 literatures were selected.The 26 included studies were methodologically assessed and analyzed by Review Manager 5.0.Fixed-effects Meta-analyses were conducted for each factor to combine odds ratio（OR）and/or relative risk（RR）outcomes across studies by study design.Overall raw point estimates of each risk factor and associated 95% confidence intervals（CI）were calculated.Results： A total of 26 studies were selected,the statistical content of the same statistics were pooled combined OR values and 95%CI of p53,FHIT,K-RAS gene mutation and smoking were caloulated p53 gene mutation in smokers was higher,showed low expression2.45-5.00）,Z=6.88,P0.0001];K-RAS gene mutation in smokers was significantly higher,Showed high expression;FHIT gene mutation in smokers was higher,showed lowexpression[OR=2.99,95%CI（1.01-8.88）,Z=1.98,P=0.005].Conclusion：The relationship between smoking and p53,FHIT,K-RAS gene mutation rate was positively correlated,in which p53,FHIT gene high expression isa protective factor,and K-RAS gene expression is a risk factor.

关 键 词：P53 FHIT K-RAS 吸烟 非小细胞肺癌 META分析 

分 类 号：R734.2[医药卫生—肿瘤]