改进的铬天青S分光光度法测定紫菜和海带中的铝  

Determination of Al in laver and kelp by modified spectrophotometric method with chrome azurol S

作　　者：杨君[1] 刁满盈[1] 贾秀莲[1] 武永平[1] 许欢[1] 

机构地区：[1]陕西省宝鸡市疾病预防控制中心,陕西宝鸡721006

出　　处：《中国卫生检验杂志》2011年第10期2408-2410,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:提出一种改进的铬天青S分光光度法检测紫菜和海带中铝含量。方法:将紫菜和海带进行预处理和湿法消化后,样品中的铝在乙酸-乙酸钠缓冲介质中,与铬天青S及溴代十六烷基吡啶形成褐紫色三元混合物,于600 nm波长处测定吸光度并与标准比较定量。结果:在所选定的最佳条件下,铝的线性回归方程y=0.0997x+0.0035,相关系数r=0.9997,方法的检出下限为0.28μg,回收率在95.6%～102.2%之间,RSD值0.49%～2.99%。结论:通过对市售样品的测定,验证了此法的准确性和精密度,适合用于测定紫菜和海带中的铝。Objective：To present a new method to determine Al content in laver and kelp by spectrophotometric method with chrome-azurol S.Methods： After pretreatment and wet digestion of samples,Al in laver and kelp reacted with Chrome Azurol S and cetyl pyridine bromide to form a ternary complex which was brown purple in acetic acid-sodium acetate buffer solution.Then the absorbance was detected at wavelength of 600nm and compared with the standard ratio.Results： The linear regression equation and correlation coefficient were y=0.0997x＋0.0035 and 0.9997 respectively.The detection limit was 0.28 μg and recovery rate was between 95.6% and 102.2%.RSD was 0.49%~2.99%.Conclusion： The method was suitable for the detection of Al in laver and kelp.The accuracy and precision of the method was verified by determination of 30 samples sold in market.

关 键 词：紫菜 海带 铝 分光光度法 

分 类 号：O657.32[理学—分析化学]