口服木糖醇对糖尿病大鼠肾脏IGF-Ⅰ表达的影响  

Effects of xylitol administrated orally on the expression of IGF-Ⅰ in the kidneys of diabetic rats

作　　者：钱宝鑫[1,2] 沈丽津[1] 李丽[1,2] 杨文[3] 何昌玉[2] 孙丽荣[1] 林来祥[1] 张媛[2] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所卫生部及天津市激素与发育重点实验室,天津300070 [2]天津医科大学基础医学院,天津300070 [3]天津市第五中心医院,天津300450

出　　处：《中国体视学与图像分析》2011年第3期289-297,共9页Chinese Journal of Stereology and Image Analysis

基　　金：天津市自然科学基金资助项目(07JCYBJC11400);天津医科大学基础医学院大学生科研基金

摘　　要：目的检测口服木糖醇对实验性1型糖尿病模型大鼠血糖及肾脏IGF-Ⅰ表达的影响。方法链脲佐菌素(STZ)一次性尾静脉注射造1型糖尿病大鼠模型,将成模大鼠随机分为糖尿病对照组(DC),糖尿病5%、10%、20%木糖醇(Xy)喂养组(D+5%、10%、20%Xy),另外设置正常对照组(NC)。喂养中分别在饲料中添加不同比例的木糖醇,开始分组喂养前留取血糖结果,第8 w末处死,处死前测量血糖。通过SABC免疫组织化学和定量分析方法观察各组大鼠肾脏IGF-Ⅰ表达的平均光密度(MOD)。结果血糖结果≥16.7 mmol/L证实模型构建成功。差异性喂养8 w后,各糖尿病组(DM)血糖与NC组相比仍升高(P<0.05),DC组血糖较0 w升高(P<0.05),而各D+Xy组8 w较0 w无明显变化。肾皮质/髓质IGF-Ⅰ表达的组间变化趋势一致:DC组较NC组IGF-Ⅰ表达量高;相比于DC组,随着饲料中添加木糖醇以及浓度的升高,IGF-Ⅰ表达量有逐渐降低趋势;皮质D+20%Xy组较DC组降低(P<0.01)且已与NC组无差异,髓质三个D+Xy组的IGF-Ⅰ表达量介于NC组、DC组之间。糖尿病大鼠在口服不同浓度木糖醇8 w后血糖与肾脏IGF-Ⅰ的表达量高度相关,r皮质=0.981(P=0.019),r髓质=0.998(P=0.002)。当血糖t∈28～35 mmol/L,皮质/髓质回归曲线方程可分别表示为y=186.036-705.932/t(R2=0.977,P=0.012),y=122.038+13.157 l nt(R2=0.997,P=0.002)。结论口服一定剂量木糖醇可稳定血糖及抑制肾脏IGF-Ⅰ表达,具有一定保护肾脏的作用。Objective To detect the effect of orally administrated xylitol on expression of IGF-I in kidneys of experimental type 1 diabetic rats.Methods The type 1 diabetic rats were developed by a single injection of streptozotocin via the tail vein.The rat models were divided into 4 groups randomly： Diabetic control（DC） group,and 5%,10%,and 20% xylitol-treated diabetic（D＋5%,10%,20% Xy） groups.In addition,a normal control（NC） group was also included.The rats were fed with feedstuff mixed with xylitol at different doses for 8 weeks.The blood glucose（BG） was recorded before the xylitol treatment and just before sacrifice.Paraffin sections of the kidney tissues were prepared and HE stained for histological examination.The expression of IGF-I in kidneys of the 5 groups was determined by SABC immunohistochemistry.Results The rats with blood glucose ≥16.7 mmol/L were considered as successful diabetic rat models.After 8 weeks of xylitol administration,the blood glucose level of the 4 DM groups were significantly higher than that in the NC group（P0.05）.The DC group had a higher blood glucose level than pre-feeding while the three D＋Xy groups did not show significant changes.The expression of IGF-I in the renal cortex and medulla showed the same variation tendency among the groups： DC groups was higher than NC groups,and the level of was in an inverse proportion to the amount of xylitol added into the feedstuff.In the renal cortex,in the D＋20% Xy group was very significantly lower than that in the DC group（P0.01） and showed no significant difference to that of the NC group.In the renal medulla,the IGF-I expression in the 3 D＋Xy groups was lower than that of the DC group,but higher than that in the NC group,showing no significant difference to either of them.The blood glucose and renal IGF-I expression of diabetic rats showed a high correlation after the 8-week xylitol treatment at different concentrations.rcortex= 0.956（P = 0.044）,rmedullar= 0.998（P = 0.002）.When the blood glucose≥28�

关 键 词：木糖醇 IGF-Ⅰ 糖尿病 大鼠 肾脏 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]