血管内皮生长因子和色素上皮衍生因子在实验性视网膜新生血管中表达  

Expression of VEGF and PEDF in experimental retinal neovascularization

作　　者：底煜[1] 盖春柳[1] 郑坤[1] 陈晓隆[1] 

机构地区：[1]中国医科大学附属盛京医院眼科,沈阳110004

出　　处：《中国实用眼科杂志》2011年第10期1091-1093,共3页Chinese Journal of Practical Ophthalmology

基　　金：辽宁省自然科学基金资助（20052089）;辽宁省科技计划项目基金资助（2010225034）

摘　　要：目的研究血管内皮生长因子（vascular endothelial growth factot，VEGF）和色素上皮衍生因子（pigment epithelium derived factor，PEDF）在实验性小鼠视网膜新生血管（retinal neovascularization，RNV）中的表达情况。探讨VEGF／PEDF比值变化与RNV形成过程的相关性。二者在RNV形成过程中所起的作用。方法以高浓度氧诱导C57BL／6J小鼠建立RNV模型。分别于出氧箱后，即鼠龄P12、P14、P17、P21时取出眼球，采用免疫组织化学法检测RNV中VEGF和PEDF蛋白的表达情况。结果VEGF和PEDF在OIR的RNV形成过程中均有显著表达。VEGF阳性表达于P14～P17最明显，P17表达最强烈，P17后表达下降；PEDF于P12表达最强烈，P12后明显下降，P17下降最明显。2种因子动态表达与RNV发展存在一定时间相关性。结论VEGF和PEDF明显表达于实验性小鼠RNV中，二者表达失衡可能参与RNV形成及发展过程的调控。Objective To study the expression of vascular endothelial growth factor （VEGF） and pigment epithelium derived factor （PEDF） in experimental retinal neovascularization （RNV） of mice. Methods RNV model was established in C57BL/6J mice exposed to oxygen of high concentration. The eyeballs were taken out at the P12, P14, P17 and P21. The expression of VEGF and PEDF in RNV was detected by immunohistochemical method. Results VEGF and PEDF were both significantly expressed in RNV of OIR. The positive immune reaction of VEGF was most apparent during P14-P17 and the expression reached the highest at P17 and reached the highest at P12 and then declined until then declined remarkably. The expression of PEDF P17 remarkably certain time correlation existed between the dynamic expressions of VEGF and PEDF and the development of RNV. Conclusions VEGF and PEDF are significantly expressed in experimental RNV. The imbalance between their expressions may play an important role in the occurrence and development of RNV.

关 键 词：血管内皮生长因子 色素上皮衍生因子 视网膜新生血管 小鼠 

分 类 号：R77[医药卫生—眼科]