局灶性脑梗死模型大鼠相对体质量、Bederson评分与梗死类型的鉴别  

Identification of a rat model of focal cerebral infarction by relative body weights and Bederson’s scale scores

作　　者：谭杰[1] 李承晏[1] 李涛[1] 张乾[1] 俞小梅[1] 袁天琪[1] 

机构地区：[1]武汉大学人民医院神经内科,湖北省武汉市430060

出　　处：《中国组织工程研究与临床康复》2011年第37期6875-6878,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:采用MCAO法制作大鼠脑缺血模型时,通过分析Bederson评分结果并不能完全可靠的诊断皮质下梗死。目的:对SD大鼠皮质梗死和皮质下基底核区梗死在不同时间点的体质量与Bederson评分结果进行比较。方法:参照ZeaLonga的线栓造模方法制作大脑中动脉闭塞模型SD大鼠,栓塞100min后拔出线栓。术后将大鼠进行磁共振成像扫描,根据有无梗死灶及梗死部位的不同将大鼠分为:皮质下基底核区梗死组(n=13)、皮质梗死组(n=25)、未出现梗死组(n=10)。对3组大鼠大脑中动脉闭塞后7周内的体质量与Bederson评分结果进行监测。结果与结论皮质下梗死组与无梗死组在各时间点大鼠相对体质量相比无显著性意义(P>0.05)。皮质梗死组大鼠的相对体质量在大脑中动脉闭塞后2周内明显小于皮质下梗死组,在大脑中动脉闭塞后3周内明显小于无梗死组(P<0.05)。皮质下梗死组大鼠造模后第1天Bederson评分结果与皮质梗死组相比差异无显著性意义,但都明显高于无梗死组(P<0.05)。提示可结合大鼠大脑中动脉闭塞后第1天的相对体质量与Bederson评分结果对大鼠的梗死类型进行鉴别。BACKGROUND：Bederson’s scale scores cannot provide reliable evidence for subcortical infarction in establishing a rat model of cerebral ischemia by middle cerebral artery occlusion （MCAO）. OBJECTIVE：To compare the body weights and Bederson scale scores at different time points in rats with two different types of cerebral ischemia. METHODS：Forty-eight male SD rats aged 7-9 weeks were included in this study. A nylon filament was inserted into the middle cerebral artery to induce a 100-minute occlusion referring to Zea Longa’s method. Then, MCAO rats were divided to three groups according to different MRI manifestations：subcortical infarction group （SIG, n=13）, cortical infarction group （CIG, n=25）, no infarction group （NIG, n=10）. Body weights and Bederson’s scale scores were monitored in all rats within 7 weeks after MCAO. RESULTS AND CONCLUSION： There was no significant difference in body weights between SIG and NIG at each time point （P 0.05）. Body weights of rats in the CIG were significantly lower than those in the SIG within 2 weeks after MCAO （P 0.05） and were significantly lower than those in the NIG at 3 weeks after MCAO （P 0.05）. There was no significant difference in Bederson’s scale score between SIG and CIG （P 0.05）, but the Bederson’s scale score in these two groups was significantly higher than that in the NIG （P 0.05） within 1 day after MCAO. Results suggest that different types of cerebral ischemia in rats can be distinguished by combining relative body weights and Bederson’s scale score within 1 day after MCAO.

关 键 词：大脑中动脉闭塞 线栓法 皮质下梗死 体质量 Bederson评分 组织工程 

分 类 号：R318[医药卫生—生物医学工程]