BDE-47对脑发育期大鼠甲状腺激素内稳态及谷胱甘肽抗氧化系统的影响  

Effects of developmental exposure to BDE - 47 on the thyroid hormone homeostasis and antioxidant system of glutathione

作　　者：鲁越[1] 冀秀玲[1] 薛赛瑜[1] 骆庆和[2] 赵文娟[2] 殷明[2] 蒋惠男[3] 张江[3] 

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海交通大学药学院,上海200240 [3]上海农林职业技术学院,上海201620

出　　处：《安全与环境学报》2011年第5期6-10,共5页Journal of Safety and Environment

基　　金：国家自然科学基金项目(20907007;30970685);教育部霍英东基金优选项目(114044);教育部新教师基金项目(20070255017);上海市重点学科开放基金项目(B604);上海市科委基金项目(09140901900)

摘　　要：以处于脑发育期的新生3 d大鼠为试验对象,研究不同剂量2,2’,4,4’-四溴联苯醚(BDE-47)对大鼠暴露21 d后,大鼠体内甲状腺激素内稳态、脑中谷胱甘肽及相关酶的变化。结果显示,与对照组相比,大鼠血清中总甲状腺激素(TT4)、游离甲状腺激素(FT)、总三碘甲状腺原氨酸(TT3)无显著改变,但高暴露剂量组大鼠血清中游离三碘甲状腺原氨酸(FT3)含量显著降低(p<0.01)。氧化损伤试验结果表明,与对照组相比,各暴露组大鼠脑中氧化型谷胱甘肽(GSSG)含量显著上升(中、低剂量组P<0.01,高剂量组p<0.05);暴露组大鼠脑中谷胱甘肽过氧化物酶(GSH-px)活力变化与剂量相关,呈现低、中剂量升高,高剂量暴露下降的趋势;暴露大鼠脑中谷氨酸半胱氨酸合成酶(GCL)活性、还原型谷胱甘肽(GSH)含量无显著变化,但GCL呈明显的下降趋势。脑发育期BDE-47暴露将造成大鼠甲状腺激素,尤其是FT3的内稳态失衡,并造成机体GSH抗氧化系统相关指标的紊乱,出现氧化应激效应。The present paper is aimed to introduce its authors＇ exper- imental findings of the effects of developmental exposure to BDE-47 on the thyroid hormone homeostasis and antioxidant system of glu- tathione. In conducting our experiments, new-born three-day old ba- by Sprague-Dawley rats were let to be exposed to different doses for 21 days to test the thyroid hormone disruptive effects and the glu- tathione related oxidative stress. The results of our experiments show that compared with the control group, no significant changes were found of the total thyroxine and free thyroxine （Fl＇4） in the rat serum. Thus, a decrease trend of the total 3,3＇ , 5-triiodothyronine was noted after the big dose exposure though not statistically signifi- cant. However, free 3,3＇ ,5-triiodothyronine （FI3） in rat serum of high-dose exposures tends to decrease significantly （p 〈 0.01 ） when compared with the control group, thus suggesting that FIB is likely to be the most sensitive subject to the thyroid disruption of BDE-47. The experimental results of induced oxidative stress also indicate that 21 - day exposure, though statistically insignificant, reveals an overall downward tendency of the activity of glutamate-cysteine ligase （GCL）, hence proving the inhibition on the GCL as a result of differ- ent doses exposure. As for the index of reduced glutathione （GSH）, no significant changes were detected in any of the exposure groups when compared with the control group. Nevertheless, the variation trend of glutathionc peroxidase （GSH - Px） remains similar as GSSG, where a significant increase was found after the exposure of 100 μg/kg BDE -47 （p 〈 0.01） but drop to the level of controls after the high dose exposure. Therefore, this study claims to find that the developmental exposure of BDE - 47 has resulted in the imbalance of thyroid homeostasis, especially for the index of FT3, which may also lead to a disorder of GSH red-ox system.

关 键 词：环境科学技术基础学科 BDE-47 甲状腺激素 氧化应激 谷胱甘肽 脑发育期 大鼠 

分 类 号：X18[环境科学与工程—环境科学] R994.6[医药卫生—毒理学]