植酸对人肝癌细胞株HepG_2细胞周期及相关蛋白表达的影响  

EFFECTS OF PHYTIC ACID ON CELL CYCLE AND THE RELATED PROTEIN EXPRESSION IN HUMAN HEPATOMA CELL LINE HepG2 IN VITRO

作　　者：李馨[1] 宋扬[2] 

机构地区：[1]青岛大学医学院细胞与分子生物学实验中心,青岛266021 [2]青岛大学医学院营养学研究所,青岛266021

出　　处：《营养学报》2011年第5期476-479,共4页Acta Nutrimenta Sinica

基　　金：山东省博士基金(No.2007BS03042)

摘　　要：目的研究肌醇六磷酸(IP6)对人肝癌细胞株HepG2细胞周期的影响并探讨其作用机制。方法以体外培养人肝癌细胞株HepG2为研究对象,应用流式细胞仪检测不同浓度IP6作用24h后对HepG2周期的影响;以免疫细胞化学法检测IP6对细胞周期相关蛋白cyclinD1、Rb、P27表达的影响;RT-PCR法检测IP6对HepG2细胞cyclinD1、CDK4 mRNA表达的影响。结果经IP6作用处理的HepG2细胞的细胞周期发生G1期阻滞。免疫细胞化学结果显示:与对照组比,各IP6浓度组均能抑制cyclinD1蛋白的表达(F=225.02,q=15.20-25.35,P<0.05),上调Rb(F=63.31,q=2.77-13.06,P<0.05)、P27蛋白的表达(F=254.75,q=4.71-25.71,P<0.05);RT-PCR结果显示,与对照组相比,各IP6浓度组均能抑制cyclinD1(F=672.34,q=16.41-41.99,P<0.05)和CDK4 mRNA(F=108.35,q=5.32-16.27,P<0.05)的表达。结论 IP6对HepG2细胞生长具有明显的抑制作用。其机制可能是IP6降低cyclinD1、CDK4水平,上调Rb、P27蛋白的水平而作用于G1-S限制点,使HepG2细胞周期发生G1期阻滞,从而起到抑制细胞增殖的作用。Objective To investigate the effects of phytic acid（IP6） on cell cycle of human hepatoma cell strain HepG2.Method HepG2 cells were exposed to various concentrations of IP6 for certain time.Flow cytometry analysis was performed for cell cycle progression.Immunocytochemical stain was used to test the expression of cyclin D1,Rb and P27 proteins.RT-PCR was used to test the expressions of cyclin D1 mRNA and CDK4 mRNA in HepG2 cells.Results IP6 could cause G1 cell cycle arrest in HepG2 cells.Different concentrations of IP6 decreased the expression of cyclin D1 protein（F=110.21;q=5.88-16.92;P0.05） and strongly increased the expressions of Rb（F=63.31,q=2.77-13.06,P0.05） and P27（F=254.75,q=4.71-25.71,P0.05）.Compared with the control group,the expressions of cyclinD1（F=672.34,q=16.4141.99,P0.05）and CDK4（F=108.35,q=5.32-16.27,P0.05）mRNA were significantly decreased at all IP6 concentrations.Conclusion IP6 had an inhibitory effect on proliferation of HepG2 cells and its mechanisms might be related to many factors,such as inhibiting the abnormal expressions of cyclinD1 and CDK4,and inducing the expressions of cell cycle inhibitor Rb and P27.

关 键 词：IP6 肝癌 HEPG2细胞 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]