二氧化氯对颤蚓成虫灭活效果及灭活作用机制  

Inactivation effect and mechanism of chlorine dioxide on adult T. tubifex

作　　者：黄廷林[1] 李晓钰[1,2] 聂小保[1,3] 张金松 李伟[1] 张爽[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055 [2]中国新时代国际工程公司,西安710055 [3]长沙理工大学水利工程学院,长沙410076 [4]深圳水务集团,深圳518031

出　　处：《环境工程学报》2011年第11期2513-2518,共6页Chinese Journal of Environmental Engineering

基　　金：国家"水体污染控制与治理"科技重大专项(2009ZX07423-003);教育部创新团队项目(IRT0853)

摘　　要：采用对苯二甲酸(terephthalic acid,TA)作为羟基自由基(hydroxyl radical,.OH)捕捉剂,利用荧光分光光度法测定了二氧化氯(ClO2)接触后颤蚓体内.OH浓度变化,结合颤蚓灭活率、过氧化氢酶(catalase,CAT)和丙二醛(malondi-alderhyde,MDA)等指标的测试结果,研究了ClO2对颤蚓成虫的灭活效果及灭活机理。结果表明,ClO2对颤蚓成虫灭活效果显著。当ClO2投加浓度超过0.4 mg/L,可对颤蚓体内.OH产生明显的诱导效应,并且.OH诱导效应与颤蚓灭活率直接相关。接触条件下CAT变化情况表明,颤蚓受到了明显的氧化胁迫,且对颤蚓体内抗氧化系统有破坏作用,MAD变化结果则表明ClO2对颤蚓的脂质过氧化作用不明显,可以对颤蚓表皮层有效渗透。上述结果证实了ClO2对颤蚓成虫的灭活作用机制之一是诱导其体内生成.OH,产生氧化胁迫,而ClO2对颤蚓抗氧化系统的破坏作用和对颤蚓表皮层的有效渗透进一步增强灭活效果。The fluctuation of ~ OH concentration in T. tubifex exposed to chlorine dioxide was investigated by fluorospeetrophotometry using terephthalic acid （TA） as · OH trapping agent. Catalase （CAT） activity in T. tubifex was quantified after 10 minutes contact with chlorine dioxide. Inactivation effect and lipid peroxidation produce malondialderhyde （MDA） of adult T. tubifex were also studied. Results showed that chlorine dioxide had significant inactivation effect on adult T. tubifex. Significant · OH inductive effect, which has direct correlation with T. tubifex inactivation effect, was observed when chlorine dioxide level was high than 0.4 mg/L. The fluctuation of CAT indicated that T. tubifex was suffering from oxidative stress and chlorine dioxide damaged the antioxidant defense system of T. tubifex. The variation of MAD showed that chlorine dioxide does not have a sig- nificant lipid peroxidation effect on adult T. tubifex and can permeate the worm＇ s cuticular layer easily. Such results confirmed that one of the inactivation mechanisms of chlorine dioxide is inducing · OH increase, which results in oxidative stress on adult T. tubifex. Moreover, chlorine dioxide can damage antioxidant defense system of T. tubifex and permeate worm＇ s euticular layer easily, which will strengthen its inactivation effect on T. tubifex.

关 键 词：颤蚓 二氧化氯 灭活 羟基自由基(·OH) 过氧化氢酶(CAT) 丙二醛(MDA) 

分 类 号：X174[环境科学与工程—环境科学] TU991.25[建筑科学—市政工程]