环王巴明对人乳腺癌细胞系MCF-7的作用  

The function of cyclopamine to human mammary carcinoma cell line MCF-7

作　　者：李锦成[1] 薛万里[1] 朱德淼[1] 陶维[1] 

机构地区：[1]辽宁医学院附属第一医院乳腺外科,辽宁锦州121001

出　　处：《中国现代医学杂志》2011年第21期2596-2599,共4页China Journal of Modern Medicine

基　　金：辽宁省教育厅高等学校科研项目(No.2008398)

摘　　要：目的探讨环王巴明对人乳腺癌细胞系M C F-7细胞增殖的影响及其分子生物学机制。方法以不同浓度的环王巴明处理M C F-7细胞不同时间,采用四甲基偶氮唑盐(M TT)分析法、流式细胞术及W esternblot检测。结果环王巴明对M C F-7细胞增殖的抑制呈浓度和时间依赖性(P<0.05)。随着药物作用时间延长,G 0/G 1期细胞的百分比明显增加而S期细胞的百分比明显减少(P<0.05);并且细胞凋亡率也随着药物作用时间的延长而增加(P<0.05)。M C F-7细胞周期素D 1的表达逐渐降低而p21表达逐渐增高(P<0.05)。结论环王巴明抑制M C F-7细胞的增殖,阻滞细胞从G 1期向S期转化,其机制可能与调控H edgehog(H h)信号通路的相关细胞周期蛋白表达有关。【Objective】 To investigate cyclopamine on proliferation of human mammary carcinoma cell line MCF-7 and its molecular biological mechanism.【Methods】 The MCF-7 cells were treated for different duration by different concentrations of cyclopamine.Methabenzthiazuron（MTT） assay,flow cytometry and western blot were used to measure.【Results】 The proliferation inhibition of cyclopamine to MCF-7 cells was concentration and time dependent（P〈0.05）.The percentage of G0/G1 proliferation phase cells signifycantly increased and that of S phase cells significantly decreased with prolongation of the drug duration（P〈0.05）.And cellular apoptosis rate of MCF-7 increased with prolongation of the drug duration.The expression level of cyclin D1 significantly decreased and the expression of p21 significantly increased with prolongation of the duration of cyclopamine（P〈0.05）.【Conclusions】 Cyclopamine significantly inhibited proliferation of MCF-7 cells and can block transformation of the cells from G1 phase to S phase.The effect of cyclopamine may be related to the expression of cyclin which are used to regulate and control Hedgehog（Hh） signaling pathway.

关 键 词：乳腺癌 MCF-7 环王巴明 细胞周期蛋白 

分 类 号：R737.9[医药卫生—肿瘤]