高效液相色谱法分析啤酒花浸膏中的6种酸性成分  

Analysis of six acidic components in hops extracts by high performance liquid chromatography

作　　者：蔡小明[1] 夏璐 孙元社 李彤 夏明珠[1] 

机构地区：[1]南京理工大学工业化学研究所,江苏南京210094 [2]大连依利特分析仪器有限公司,辽宁大连116023 [3]辽宁大连依利特分析仪器工程技术研究中心,辽宁大连116023

出　　处：《色谱》2011年第10期983-987,共5页Chinese Journal of Chromatography

基　　金：江苏省科技支撑计划社会发展项目(No.BE20100731);辽宁省科学技术计划项目(No.201002703);大连市科学技术基金计划项目(No.2009J22DW015)

摘　　要：建立了啤酒花浸膏中6种酸性成分(合葎草酮、葎草酮、加葎草酮、合蛇麻酮、蛇麻酮、加蛇麻酮)的高效液相色谱分析方法。分别考察了酸的加入、有机相种类及柱温对色谱分离效果的影响。在室温条件下,以HypersilODS2柱(250 mm×4.6 mm,5μm)为分析柱,以乙腈-0.1%(v/v)磷酸水溶液(pH 2.2)(65∶35,v/v)为流动相,在1.0 mL/min流速下等度洗脱,于315 nm波长下检测,啤酒花浸膏中6种酸性成分在等度洗脱下实现了基线分离。收集6种酸性成分,分别通过紫外光谱、红外光谱及质谱对其结构进行表征。结果表明该方法具有稳定、简便的优点,适用于啤酒花浸膏中酸性成分的分析。A method of high performance liquid chromatography（HPLC） was developed for the separation and determination of six acidic components（cohumulone,humulone,adhumulone,colupulone,lupulone and adlupulone） in hops extracts.The effects of several important factors,such as the addition of acid,the organic solvent of elution solution and the column temperature,were investigated to acquire the optimum conditions.The separation was carried out on a Hypersil ODS2 column（250 mm×4.6 mm,5 μm）.A mixture of acetonitrile-0.1%（v/v） phosphoric acid solution（pH 2.2）（65∶35,v/v） was used as the mobile phase at a flow rate of 1.0 mL/min in isocratic elution mode.The column temperature was kept at room temperature,and the detection wavelength was set at 315 nm.The six acidic components reached baseline separation,and were identified by ultraviolet spectroscopy,infrared spectroscopy and mass spectrometry.The results show that this method is suitable for the analysis of acidic components in hops extracts owing to the stable and simple performance.

关 键 词：高效液相色谱 合葎草酮 葎草酮 加葎草酮 合蛇麻酮 蛇麻酮 加蛇麻酮 啤酒花浸膏 

分 类 号：O658[理学—分析化学]