枸杞提取物对MPP+诱导的线虫和PC12细胞神经毒性的保护作用  

Protective Effects of Lycium barbarum Extract Against MPP+-induced Neurotoxicity in Caenorhabditis elegans and PC12 Cells

作　　者：姚晓黎[1] 吴婉玲[1] 郑民缨[1] 李伟[1] 叶城辉[1] 卢锡林[1] 

机构地区：[1]中山大学附属第一医院神经科,广东广州510080

出　　处：《中药材》2011年第8期1241-1246,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然基金资助项目(81041056);广东省科技计划项目(2010B050700024)

摘　　要：目的:观察枸杞提取物对MPP+诱导的线虫和PC12细胞神经毒性的保护作用并探讨其作用机制。方法:以不同浓度枸杞提取物预处理MPP+诱导的线虫和PC12细胞多巴胺能神经损伤模型,计算线虫的存活率和选择性表达绿色荧光的多巴胺能退行性变神经元的数量,以MTT法和核形态检测PC12细胞的损伤,检测PC12细胞的胞内的氧自由基、线粒体膜电位、总GSH水平。结果:枸杞提取物明显提高了MPP+处理过的线虫的存活率和保护了多巴胺能神经元,对MPP+诱导的PC12细胞的具有明显的保护作用,作用呈量效关系。枸杞提取物通过减少MPP+诱导PC12细胞胞内ROS的过量积聚,减少线粒体损伤,恢复GSH水平而发挥其保护作用。结论:枸杞提取物主要通过抗氧化及恢复GSH水平而发挥其对MPP+诱导的线虫和PC12细胞神经毒性保护作用。Objective：To investigate the neuroprotective effects of lycium barbarum extract against MPP＋-induced neurotoxicity in Caenorhabditis elegans and PC12 cells and its mechanism.Methods： Pretreated MPP＋-induced nearotoxicity in C.elegans and PC 12 cells with Lycium barbarum at differert dosages.The viability and DA neurodegeneration was assessed in C.elegans selectively expressing green fluorescent protein（GFP） in DA neurons.PC12 cell damage was measured using MTT and nuclear morphology.Intracellular reactive oxygen species（ROS）,mitochondrial membrane potential and total GSH were assessed.Results：Lycium barbarum extract protected against MPP＋-induced loss of viability and DA neurodegeneration in C.elegans in a dose-dependent manner.Similar neuroprotection was replicated in MPP＋ PC12 cell model.Lycium barbarum extract attenuated MPP＋-induced intracellular ROS accumulation,loss of mitochondrial membrane potential and restored total GSH levels in PC12 cells.Conclusions：Lycium barbarum extract protects against MPP＋-induced neurotoxicity in C.elegans and PC12 cells and its machanism may be related to its antioxidative property and restoration of total GSH level.

关 键 词：枸杞提取物 MPP+线粒体 线虫 总GSH 氧自由基 

分 类 号：R285.5[医药卫生—中药学]