高浓度胆汁改变肠上皮细胞氯通道蛋白-2和通透性  

High concentration bile changes the cldoride channel protein-2 and permeability of intestinal epithelium cell line

作　　者：陈振勇[1] 张劲[1] 杨鹏[1] 黄文广[1] 冯贤松[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院综合外科,武汉430022

出　　处：《中华实验外科杂志》2011年第11期1858-1860,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察胆汁对肠上皮细胞氯离子通道和通透性的影响。方法鼠肠上皮细胞株IEC-6分别与5．0％、1．0％、0．1％胆汁及氯通道激动剂接触。20h后检测跨膜电阻，Western blot分析氯离子通道蛋白-2（CLC-2）和紧密连接闭锁小带-1（ZO-1）表达的变化及各条带的相对灰度值。结果5．0％浓度组降低跨膜电阻作用最强。5．0％和1．0％组的CLC-2蛋白相对灰度值（0．30±0．05和0．37±0．08）低于对照组（P均〈0．05）。5．0％组的ZO-1相对表达量下降。添加氯通道激动剂后，5．0％浓度组的跨膜电阻（451．3±60．5）Ω·cm^2及ZO-1相对表达量（0．32±0．04）较对照组上升明显（P均〈0．05）。结论高浓度胆汁破坏肠上皮细胞氯离子通道和紧密连接蛋白，增加上皮细胞通透性。Objective To investigate the effects of bile on chloride channel protein-2 （CLC-2） and permeability of intestinal epithelium cell. Methods Rat intestinal epithelium cell line IEC-6 was cultured. They were exposed to 5.0%, 1.0%, 0. 1% rat bile and chloride channel agonist Lubiprostone. After 20 h cultured, transepithelial electrical resistance （TER） of the monostratal cells was measured. The change of CLC-2 and zannla occludens-1 （ ZO-1 ） were examined by Western blotting, and the images were analysed quantitatively. Results The TER of 5% group was most lower. The average relative gradations of Western blotting images in 5.0% group and 1. 0% were lower obviously than those in control group （0. 30 ±0.05, 0. 37 ±0. 08 vs. 0. 56±0. 08） （ all P 〈 0.05 ）. The relative gradations of ZO-1 were decreaseded only in 5.0% group. After Lubiprostone added, the TER in 5.0% group was upgraded （451.3 ± 60. 5 ） Ω·cm^2, and the average relative gradations of ZO-1 （0. 32 ± 0. 04）. Conclusion High concentration of bile destroied enterocyte chloride channel and tight junction protein, increase epithelium permeability.

关 键 词：胆汁 肠上皮细胞 氯离子通道蛋白.2 肠通透性 

分 类 号：R575[医药卫生—消化系统]