人类快速延迟整流性钾通道蛋白与血管内皮生长因子在胃癌组织中的表达及其相互关系  

HERG1 protein and vascular endothelial growth factor expression in gastric cancersand their correlation

作　　者：吕清[1] 王国斌[1] 卢晓明[1] 孟庆良[1] 舒晓刚[1] 肖勇[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科,武汉430022

出　　处：《中华实验外科杂志》2011年第11期1906-1908,I0002,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察人类快速延迟整流性钾通道基因表达的钾离子通道蛋白（HERG1）和血管内皮生长因子（VEGF）在胃癌组织中的表达，探讨两者与胃癌预后的关系。方法应用免疫组织化学方法（SP法）、逆转录-聚合酶链反应（RT—PCR）技术方法对80例胃癌组织标本及对应正常胃黏膜组织中的HERG1蛋白和VEGF的表达进行检测，并进行回顾性随访。结果免疫组织化学、RT—PCR检测胃癌组织中HERGl的阳性表达率分别为60％、90％，VEGF的阳性表达率分别为55％、85％；HERG1蛋白表达与淋巴结转移，远处转移关系密切；VEGF的表达与胃癌的浸润程度、TNM分期、分化程度、淋巴结转移明显相关（P〈0．05），而与性别、肿瘤大小、部位无明显相关。结论HERG1蛋白和VEGF的过度表达与胃癌的发生发展有关，在肿瘤侵袭、转移过程中起重要作用，其共位表达有助于判断胃癌预后。Objective To investigate the expression of HERG1 protein and vascular endothelial growth factor （VEGF） in gastric carcinomas and the correlation between HERG K^＋ channels and VEGF. Methods Reverse transcription and PCR （RT-PCR） and immunohistochemistry （IHC） were used to detect the expression of HERG1 gene and VEGF in 80 gastric carcinomas and the patients were followed-up. Results The positive expression of HERG1 and VEGF was detected in 48 cases （60%） and 44 cases （55%） by immunohistochemistry, and in 72 cases （90%） and 70 cases （85%） by RT-PCR, respectively. There was a significant correlation between the positive expression of VEGF and depth of invasion, TNM stage, differention, lymphatic and distant matastasis in gastric carcinomas （P 〈 0.05 ）. The positive expression of HERG1 was correlated with lymphatic and distant metastasis. Conclusion HERG K^＋ channels and VEGF overexpression in gastric carcinomas appeared to be correlated with tumor genesis and development, and may play an important role in the tumor invasion and metastasis. The co-expression of HERG K ＋ channels and VEGF could be well-characterized indicator to prognosis of gastric cancer.

关 键 词：胃肿瘤 转移 HERG1蛋白 基质金属蛋白酶-9 

分 类 号：R735.2[医药卫生—肿瘤]