健脾益肾活血法对ITP模型小鼠细胞因子的影响  

Effect of Jiangpi Yishen and Huoxue Treatmen Principle on Cell Factors of ITP Model Mice

作　　者：黄霞[1] 庆慧[1] 刘惠霞[1] 孙为[1] 

机构地区：[1]河南省中医药研究院,河南郑州450004

出　　处：《辽宁中医药大学学报》2011年第11期9-11,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：河南省科技攻关项目(082103810805)

摘　　要：目的:观察健脾益肾活血法(简称JYH)对ITP模型小鼠细胞因子的影响。方法:BALB/c小鼠随机分为空白对照组、模型对照组、PA对照组和JYH低、中、高剂量组,各组连续灌胃14天。于实验第5天开始,除空白对照组外,各组连续3天腹腔注射豚鼠抗小鼠血小板血清(GP-APS)复制ITP模型。电阻抗法检测外周血PLT数量,放免法检测血清IL-2、IL-6水平。结果:与空白对照组相比,各组小鼠PLT数量明显减少(P<0.05),治疗各组PLT检测值均高于模型对照组,PA对照组和JYH高剂量组与模型对照组相比差异显著(P<0.05);各组造模小鼠血清IL-2水平均高于空白对照组而IL-6水平则低于空白对照组,JYH治疗组可下调模型小鼠血清IL-2水平并拮抗血清IL-6下降(P<0.05)。结论:健脾益肾活血法对ITP小鼠细胞因子水平失衡具有改善作用。Objective：To observe the effect of Jiangpi Yiqi and Huoxue treatment principle on cell factor of ITP model mice.Methods：The Balb/c mice were randomly divided into 6 groups（Normal control group,Model control group,PA control group,JYH low,middle and high dose groups）.The mice were continuously given irrigated gastrom for 14 days.Test on the fifth day,the mice were injected with Guinea pigs anti mice platelet serum（GP-APS）solution into the celiac of mice for duplicating the ITP model except normal control group.The Platelet（PLT）quantity in peripheral blood were detected by Resistance.The levels of IL-2 and IL-4 in serum were detected by radioimmunoassay.Results：The PLT was significantly lesser in model groups than that in normal group（P〈0.05）,The PLT of mice in treatment groups were higher than that in model group,among the rest the PA control group and JYH high dose group had significant difference（P〈0.05）;The level of IL-2 was significantly higher and the levels IL-6 was lower in model groups than that in normal group,the treatment groups of JYH can down regulation the levels of IL-2 and raise the levels of IL-6 in model mice（P〈0.05）.Conclusions：Jiangpi Yishen and Huoxue treatmen principle had improved role on the cell factor levels unbalance of ITP model mice.

关 键 词：血小板减少性紫癜 健脾益肾活血法 小鼠 免疫药理 

分 类 号：R-322[医药卫生]