白英水提物对胃癌细胞凋亡与Notch1信号通路的影响  

Effects of Solanum Lyratum Thumb Extract on Notch1 Gene Expression and Apoptosis in Human Stomach SGC-7901 Cells

作　　者：王美凤[1] 万慧芳[2] 余乐涵[2] 涂硕[2] 万福生[2] 

机构地区：[1]南昌市第一医院,江西南昌330008 [2]南昌大学医学院,江西南昌330006

出　　处：《辽宁中医药大学学报》2011年第11期82-85,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江西省科技攻关重点项目(060004);江西省卫生厅资助项目(2010A149)

摘　　要：目的:观察白英水提物对胃癌SGC-7901细胞凋亡及Notch1基因表达的影响。方法:常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5、25mg/mL和50mg/mL。MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡,半定量RT-PCR和免疫化学分析细胞中Notch1、caspase-9基因表达。结果:与正常对照组比较,白英处理组SGC-7901细胞增殖有显著性下降,细胞凋亡显著性增多(P<0.05),Notch1基因表达呈显著性的下降(P<0.05)、caspase-9基因表达有明显的上调(P<0.05),并随白英浓度增加而加强。结论:白英水提物能下调人胃癌SGC-7901细胞中Notch1信号蛋白表达和促进细胞凋亡,这可能是其发挥抗癌作用的重要机制之一。Objective：To investigate the effects of Solanum Lyratum Thunb Extract（SLT）on apoptosis and the expression of Notch1,Caspase-9 genes in Human stomach cancer SGC-7901 cells.Methods：Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human Stomach Cancer SGC-7901 Cells were randomly divided into the control group and SLT-treated groups（12.5mg/mL,25mg/mL,50mg/mL）,the growth inhibitory rate was evaluated by MTT assay,cell apoptosis rate was determined by flow cytometry;Expression of Notch1,Caspase-9 mRNA were detected by semi-quantitive RT-PCR,Expression level of Notch1 and Caspase-9 proteins were detected by two-step immunhistochemical staining.Results：Compared with control group,the proliferation inhibitory rate and apoptosis rate were increased obviously（P〈0.05）,the expression of Notch1 mRNA and protein were obvious decreased respectively（P〈0.05）,the expression of Caspase-9 mRNA and protein were obvious increased respectively（P〈0.05）,and display a effect in a dose-dependent manner in the SGC-7901 cells of the SLT-treated groups.Conclusion：SLT can downregulate expression level of Notch1 and induce apoptosis of the Human stomach cancer SGC-7901 cells.

关 键 词：白英 NOTCH1基因 胃癌细胞 凋亡 信号通路 基因表达 

分 类 号：R222.19[医药卫生—中医基础理论]