复方藤梨根制剂对人胃癌裸鼠腹腔移植瘤的作用机制研究  

Experimental Study on Mechanism of Fufang Tengligen Mixture Inhibiting Human Gastric Cancer Implanted in Abdominal Cavity of Nude Mic

作　　者：何好臣[1] 秦建领[2] 郭勇[3] 

机构地区：[1]中国中医科学院西苑医院体检中心,北京100091 [2]郑州市第三人民医院肿瘤科,河南郑州450000 [3]浙江省中医院肿瘤科,浙江杭州310053

出　　处：《辽宁中医药大学学报》2011年第11期88-90,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：浙江省中医药管理局资助项目(A2005Y012)

摘　　要：目的:研究复方藤梨根制剂(FFTLG)对人胃癌裸鼠腹腔移植瘤的抑制效应及其作用机制。方法:建立人胃癌(SGC-7901)裸鼠腹腔移植模型。24只裸鼠随机分为4组:FFTLG高、中、低剂量组和生理盐水对照组,分别观察荷瘤裸鼠腹腔移植瘤的生长情况,免疫组化检测肿瘤基质金属蛋白酶-9(MMP-9)的表达情况。结果:NS组、FFTLG低、中、高剂量组抑瘤率分别为0、7.8%、30.0%和33.3%;实验组与模型对照组相比具有统计学意义(P<0.05,P<0.01);腹腔瘤组织中MMP-9阳性表达细胞百分比分别为(56.13±23.26)%、(45.83±13.40)%、(30.67±11.50)%、(17.53±12.50)%。实验组与模型对照组相比具有统计学意义(P<0.05,P<0.01)。结论:FFTLG对裸鼠腹腔移植瘤生长具有抑制作用,且随着剂量的增加其作用增强,其作用机制可能与下调MMP-9的表达有关。Objective：To investigate the effects and mechanism of Fufang Tengligen Mixture inhibiting human gastric cancer implanted in abdominal cavities of Nude Mice.Methods：Established models of Nude Mice with human gastric cancer transplanted in their abdominal cavities.24 Nude Mice were randomly divided into 4 groups：groups by high,moderate,low thickness FFTLG mixture and group by NS,Observed the growth of tumour in their abdominal cavities,detected the expressions of MMP-9 in gastric cancer tissue by immunohistochemistry.Results：The tumour inhibition rates of NS,low,moderate,high thickness FFTLG mixture group were 0,7.8%,30.0% and 33.3%.Compared to control group,experimental groups showed significant differences（P〈0.05,P〈0.01）;Expression rates of MMP-9 were（56.13±23.26）%,（45.83±13.40）%,（30.67±11.50）%,（17.53±12.50）%.Compared to control group,experimental groups showed significant differences（P〈0.05,P〈0.01）.Conclusion：FFTLG Mixture can inhibit the growth of tumours transplanted in abdominal cavities of Nude Mice,the more dosage,the better effect,whose mechanism may partly be to lower the expression of MMP-9.

关 键 词：复方藤梨根制剂 胃癌 裸鼠 基质金属蛋白酶-9 腹腔移植瘤 

分 类 号：R735.2[医药卫生—肿瘤]