原子力显微镜对甲状腺滤泡上皮细胞抗体结合后细胞膜表面形态结构的观察  

Useing atomic force microscope to obsevte the cell membrane surface morphology of thyroid follicular epithelial cells with antibodies

作　　者：王牧[1] 纪小龙[2] 

机构地区：[1]辽宁医学院研究生院北京武警总医院校外基地,北京100039 [2]武警总医院病理科,北京100039

出　　处：《中国医学工程》2011年第9期23-25,共3页China Medical Engineering

摘　　要：目的运用原子力显微镜(AFM)观察甲状腺滤泡上皮细胞抗体结合后的细胞膜表面结构的变化,并分析有无抗体结合的异同特点。方法以甲状腺滤泡上皮细胞为研究对象,采用细胞印片法获取各组甲状腺细胞,分别标记Villin抗体和CK7抗体为实验组及滴加PBS缓冲液的对照组三个试验组(每组各300个)。应用AFM(轻敲模式)扫描各组细胞并利用件自动分析系统进行分析。结果 (1)AFM扫描后图像分析结果:未加抗体的对照组甲状腺滤泡上皮细胞表面的隆起较光滑,分布均匀、形状规则,排列疏密适中;Villin抗体组细胞表面的隆起较对照组突起与凹陷无明显变化;CK7抗体组细胞体积稍变大,部分隆起处高度也增加,突起数量明显增多,隆起分布不均匀,沟裂处平均深度较对照组细胞浅。(2)各组细胞膜表面重要参数比较结果:各组细胞膜表面的平均粗糙度、平均峰高度、平均凹陷深度和表面积差值的比较,对照组和Villin抗体免疫组化组无统计学意义(P>0.05),对照组和CK7抗体免疫组化组具有统计学意义(P<0.01或P<0.05)。结论AFM从纳米级水平为我们提供了一条临床诊断的新思维,滴加抗体后细胞表面抗原与抗体结合,其细胞膜表面形态结构上存在显著差别,为以后的免疫组化纳米级研究奠定基础。【Objective】 To observe the changes of the structure of the cell membrane surface of thyroid follicular epithelial cells combine with antibodies by using atomic force microscope（AFM）,and to analyze the similarities and differences of with and without antibodies.【Methods】 Setting thyroid follicular epithelial cells as the object of study,getting each group of thyroid cells with the cells printing method and divided them into three groups（each group 300）,as experimental group,which are respectively marked with Villin antibodies and CK7 antibodies,and we compare them with samples which are added with PBS buffer liquid as control group.Using AFM（tapping mode） scanning cells of each group and analysising with the software automatical analysis system.【Results】（1） the AFM scanning image analysis results： The thyroid follicular epithelial cells＇surface in PBS control group is smooth,distribute uniformly,regular shape.The density of the rules are moderate;The surface of cells in Villin antibody group have no obvious changes on cells＇ surface by caompareing with the control group;CK7 antibody group cell＇s volume is slightly bigger,some bumps are more higher,the number of the bumps are increase and the bumps are distribute uneven.The crap average depth is less deeper than the control group.（2） The results of each cell membrane surface important parameters comparison： the contract of each of the cell membrane surface average roughness,average peak height,average depth and surface proportion diference.There is no statistically significant of the comparison between the control group and Villin antibody group（P 0.05）,and the comparison of control group and CK7 antibody group has statistically significant（P 0.01 or P 0.05）.【Conclusion】 AFM provides a new methord of clinical diagnosis at the nanoscale level,after the surface antigen combine with the antibody,the morphological structure of cell surface exist a significant difference,witch will form a foundation for the future im

关 键 词：原子力显微镜 甲状腺细胞 抗体结合 

分 类 号：R361[医药卫生—病理学]