自体脑外转道模式下青霉素致痫大鼠海马C-fos、C-jun表达的影响  

Expression of C-fos,C-jun in Hippocampus under the Model of Transmitting Epileptic Discharge from Brain Tissue to Muscular Tissue on Its Own Skull

作　　者：郑文[1] 李卓[1] 黄献[1] 王振[1] 徐伟[1] 宋治[1] 

机构地区：[1]中南大学湘雅三医院神经内科,长沙410013

出　　处：《生物医学工程学杂志》2011年第5期1014-1018,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30971534);湖南省自然科学基金资助项目(06JJ50034)

摘　　要：观察在脑内置入引导电极接自体脑外肌肉组织对青霉素致痫大鼠行为学、脑电图(EEG)及海马C-fos、C-jun的表达,探讨其对大鼠痫性发作的影响。实验动物随机分为三组,每只6只,分别为致痫组(海马内微注射青霉素制备痫性发作模型)、脑电转道组(致痫灶置入引导电极并接自体脑外肌肉组织)及对照组(脑内置入引导电极,不致痫)。观察各组大鼠行为学、EEG变化并应用免疫组化方法检测海马C-fos、C-jun的表达。实验中观察到致痫组、脑电转道组大鼠痫性发作次数无显著差别;脑电转道组EEG放电次数较致痫组显著减少(P<0.05);4 h点脑电转道组注药侧海马CA3、CA1区C-fos、C-jun表达明显低于致痫组注药侧(P<0.05)。本实验证实了自体脑外转道模式下,引导电极置入减少了致痫大鼠痫性放电次数,下调了致痫大鼠海马C-fos、C-jun的表达。The objective of this study was to investigate the changes of the behaviors, EEG and expression of c-fos,cjun on induced seizure in rats by injecting penicillin after transmitting epileptic discharge from brain tissue to muscu- lar tissue on skull. Eighteen experimental rats were divided into 3 groups, with each 6 rats. Seizure group： 6 acute seizure models were established by injecting penicillin in hippoeampus of rats; Transferring group： 6 acute seizure models were established by injecting penicillin in hippocampus of rats, and electrode connected to muscles was planted into epileptic focus of each rat; Control group：6 rats were only planted electrode in hippocampus without injecting penicillin. Then we observed the changes of behaviors, EEG and expression of C-los, C-jun in hippocampus with im- munohistoehemical method. There was no statistic difference in seizure frequency of rat between seizure group and transferring group, but the discharging frequency in EEG of transferring group lowered significantly （P〈0.05）. The expression of C-los, C-jun in hippocampus of transferring group rats was significantly lower than that of seizure group （P〈0. 005）. It could be concluded that under the model of transmitting epileptic discharge from brain tissue to muscular tissue on skull, the burst times on EEG electrode decreased, concomitantly with the lower expression of C-los, C-jun in hippocampus.

关 键 词：癫痫 脑电图 转道 电极 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R741.044[医药卫生—临床医学]