心肌干细胞移植可改进心力衰竭大鼠室颤阈值和电生理的稳定性  

Improvements of ventricular fibrillation threshold and cardiac electrophysiological stability in myocardial infarction following cardiac stem cells transplantation

作　　者：王彤[1] 郑韶欣[1] 周长青[1] 翁胤仑[1] 温主治[1] 黄辉[1] 王景峰[1] 

机构地区：[1]中山大学孙逸仙纪念医院急诊科,广东省广州市510120

出　　处：《中国组织工程研究与临床康复》2011年第36期6753-6756,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(81070125)"抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究";广东省科技计划项目(2010B031600032)"抗凋亡与促血管生成miRNA-378干预MSCs治疗心梗后心衰的机制研究";广东省自然科学基金(8151008901000119)"骨髓间充质干细胞在心肌梗死后重建收缩功能的机制研究"~~

摘　　要：背景:心肌干细胞移植后是否引起或加重心肌梗死患者的心律失常是治疗的关键问题。目的:观察心肌干细胞对心力衰竭大鼠室颤阈值和电生理稳定性的影响。方法:开胸结扎20只SD大鼠左前降支冠状动脉,2周后选取其中的10只局部梗死心肌内注射PKH26荧光标记的由PBS悬浮的心肌干细胞,另10只局部梗死心肌内注射等量PBS。治疗6周后,再次开胸检测梗死边缘区的心电生理特性和室颤阈值。实验结束后,摘取心脏行病理切片,检查PHK26标记的心肌干细胞是否在梗死区内生存并表达连接蛋白43及α-肌动蛋白。结果与结论:与对照组相比,心肌干细胞移植组单极电图激动恢复时间及纠正的激动恢复时间明显缩短,电刺激所激发的恶性心律失常明显减少,室颤阈值明显提高。PHK26标记的心肌干细胞在梗死边缘区内被发现并表达连接蛋白43和α-肌动蛋白。提示心肌干细胞移植能改善心力衰竭大鼠心电生理的稳定性和提高室颤阈值;心肌干细胞可在体内表达心肌细胞和连接蛋白的标记。BACKGROUND：Whether transplanted cardiac stem cells（CSCs） can improve ventricular fibrillation threshold（VFT） and whether severe malignant ventricular arrhythmia is induced in the myocardial infarction model are still unclear.OBJECTIVE：To clarity the electrophysiological characteristics and VFT in rats with myocardial infarction by treatment with allogenic CSCs.METHODS：Left anterior descending coronary arteries of SD rats were ligated to induce myocardial infarction.Two weeks later,animals were randomized to receive 5×106 CSCs labeled with PKH26 in phosphate buffer solution（PBS） or PBS alone as a placebo by injection into the ischemic zone in the anterior ventricular free wall.Six weeks after the CSCs or PBS injection,electrophysiological characteristics and VFT were measured at the infarct area.Labeled CSCs were observed in 5 μm cryostat sections from each harvested heart.RESULTS AND CONCLUSION：Compared with the PBS group,the unipolar electrograms activation recovery time dispersions were shorter in the CSCs group,malignant ventricular arrhythmias were significantly less inducible in the CSCs group,the VFTs were greatly improved in the CSCs group.Labeled CSCs were identified in the infarct marginal zone and expressed Connexin-43 and α-sarcomeric actin.CSCs can modulate the electrophysiological abnormality and improve the VFT in rats with myocardial infarction treated with allogenic CSCs and CSCs express muscle and connexin markers in vivo.

关 键 词：心肌干细胞 心律失常 心电生理学特性 室颤阈值 心肌梗死 

分 类 号：R394.2[医药卫生—医学遗传学]