体外诱导羊膜来源间充质干细胞分化为血管内皮细胞  

In vitro differentiation of human amniotic mesenchymal stem cells into vascular endothelial cells

作　　者：施晶晶[1] 朱栋晓[2] 金伟东[1] 王晓彦[1] 庄瑞娟[1] 李拜红[1] 苗宗宁[3] 

机构地区：[1]无锡市第三人民医院心内科,江苏省无锡市214041 [2]无锡市第三人民医院心超室,江苏省无锡市214041 [3]无锡市第三人民医院研究所,江苏省无锡市214041

出　　处：《中国组织工程研究与临床康复》2011年第36期6785-6788,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:羊膜来源间充质干细胞植入机体不同类型组织后是否可以分化为相应组织靶细胞呢?目的:检测血管内皮细胞生长因子在体外诱导人羊膜间充质干细胞分化为血管内皮细胞的可行性。方法:分离培养羊膜间充质干细胞,鉴定其表面抗原表达,用含体积分数2%胎牛血清以及50μg/L血管内皮生长因子的条件培养基诱导,诱导后细胞通过内皮细胞标志物血管内皮生长因子受体2以及v-WF染色鉴定。结果与结论:羊膜间充质干细胞表面抗原CD29、CD44、CD105阳性,CD34、CD45、CD106以及HLA-DR阴性。诱导后细胞形态明显改变,内皮细胞标志物血管内皮细胞生长因子受体2以及v-WF染色结果阳性。提示羊膜间充质干细胞在体外具有分化为血管内皮细胞的能力。BACKGROUND：Whether human amniotic mesenchymal stem cells can differentiate into corresponding target cells after transplantation into different types of tissues？ OBJECTIVE：To investigate the feasibility of differentiation from human amniotic mesenchymal stem cells into vascular endothelial cells in vitro induced by vascular endothelial cell growth factor.METHODS：Human amniotic mesenchymal stem cells were isolated,and then characterized by flow cytometry using a panel of monoclonal antibodies.Differentiation into endothelial-like cells was induced by cultivation of confluent cells in the presence of 2% fetal calf serum and 50 μg/L vascular endothelial growth factor.We characterized these cells by immunofluorescence staining for endothelial-specific markers KDR and the von Will brand factor.RESULTS AND CONCLUSION：Isolated human amniotic mesenchymal stem cells were positive for the markers CD105,CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34,CD45,CD106 and HLA-DR.The morphology of induced cells was obviously changed.Immunofluorescence staining analysis of the confluent cells in situ showed positive for endothelial-specific markers like KDR and the von Will brand factor.The human amniotic mesenchymal stem cells have the capability to differentiate into vascular endothelial cells in vitro.

关 键 词：羊膜 间充质干细胞 移植 血管内皮细胞 分化 

分 类 号：R394.2[医药卫生—医学遗传学]