DC-CIK细胞体外抗白血病K562细胞的免疫效应  

Immune Effect of DC-CIK Cells against Leukemia K562 Cells In Vitro

作　　者：艾丽梅[1] 宋盈盈[1] 苏荣英[1] 

机构地区：[1]辽宁医学院附属第一医院血液科,辽宁锦州121001

出　　处：《中国生物制品学杂志》2011年第10期1168-1170,1176,共4页Chinese Journal of Biologicals

基　　金：辽宁省教育厅科技研究项目(2008390);辽宁医学院博士启动基金(20090715)

摘　　要：目的探讨细胞因子诱导的杀伤细胞(Cytokine-induced killer cell,CIK)与树突状细胞(Dendritic cell,DC)共同培养后获得的DC-CIK细胞体外抗白血病细胞株K562的免疫效应。方法分离正常人外周血单个核细胞(PBMC),在细胞因子的作用下诱导成DC和CIK细胞,将两种细胞共同培养3 d获得DC-CIK细胞,MTT法检测细胞增殖活性;流式细胞术检测细胞表型;以共培养3 d的DC-CIK作为效应细胞,以PBMC、DC、CIK细胞单独培养作为对照,K562细胞作为靶细胞,采用MTT法检测各组细胞的杀伤活性,ELISA法检测各组细胞培养上清中白细胞介素-17(IL-17)水平,并分析IL-17与杀伤细胞间的相关性。结果 DC-CIK细胞增殖最快;收获的细胞大部分以成熟DC为主,CIK细胞主要是具有杀伤作用的淋巴细胞;DC-CIK细胞杀伤K562细胞的活性和分泌IL-17的水平均明显高于对照组(P<0.05),且随着IL-17释放量的增加,细胞毒性增强。结论 DC-CIK细胞通过分泌细胞因子的作用杀伤白血病细胞。Objective To investigate the immune effect of DC-CIK cells,obtained by co-culture of cytokine-induced killer（CIK） cells and dendritic cells（DCs）,against leukemia K562 cells in vitro.Methods Normal human peripheral blood mononuclear cells（PBMCs） were isolated and induced with cytokines to obtain DCs and CIK cells which were co-cultured for 3 d.The obtained DC-CIK cells were determined for proliferative activity by MTT method and for phenotype by flow cytometry,then used as effecter cells,while PBMCs,DCs and CIK cells as controls,and K562 as target cells.The cells in various groups were determined for killing activity by MTT method,and for IL-17 level in culture supernatant by ELISA.Results Compared with PBMCs,DCs and CIK cells,DC-CIK cells proliferated rapidly.The harvested cells after co-culture were mainly DCs,in which CIK cells were mainly lymphocytes with killing effect.The killing activity to K562 cells,as well as secreted IL-17 level,of DC-CIK cells were significantly higher than those of cells as controls（P 0.05）.Conclusion DC-CIK showed killing effect on K562 cells through cytokine secretion.

关 键 词：DC-CIK细胞 白血病 K562细胞 免疫效应 

分 类 号：R733.7[医药卫生—肿瘤]