晚期糖基化终产物受体RAGE在宫颈鳞癌组织中的表达及临床意义  

Expression of RAGE Protein in Human Cervical Squamous Cell Carcinoma

作　　者：付欣[1] 王慧玉[1] 田菁[1] 郝权[1] 

机构地区：[1]天津医科大学附属肿瘤医院盆腔肿瘤科,天津市肿瘤防治重点实验室,天津市300060

出　　处：《中国肿瘤临床》2011年第19期1201-1204,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨宫颈鳞癌组织中晚期糖基化终产物受体(RAGE)表达情况及其与肿瘤分化程度、大小、分期、浸润和转移的关系。方法:应用免疫组织化学方法检测了30例宫颈原位癌、90例无转移的宫颈癌、30例有转移的宫颈癌和30例正常宫颈鳞状上皮中RAGE表达情况,并应用Western blot技术定量检测其表达情况。结果:RAGE在宫颈鳞癌组织中呈强阳性表达48.0%(72/150),正常宫颈鳞状上皮中弱表达13.3%,差异具有显著性(P<0.01),RAGE的高表达与宫颈鳞癌的发生、分期、侵袭和转移有关(P<0.01),而与其组织分化程度、大小无关(P>0.05)。结论:RAGE在宫颈鳞癌组织中尤其是发生转移的宫颈鳞癌组织中呈强阳性表达,可作为宫颈鳞癌浸润、转移的重要判定指标之一,并有望成为基因治疗的靶点。Objective： To investigate the expression of the receptor of advanced glycosylation end-products （ RAGE ） in humancervical squamous cell carcinoma （ CSCC ） and to explore the relationship of this expression to several factors such as differentiation degree, size and stage of CSCC, and invasion, as well as metastasis of CSCC. Methods： Immunohistochemical staining of tissue microarrays was conducted to detect the expression of RAGE in the following tissue samples： 30 carcinomas in situ, 90 invasive CSCCs without metastasis, 30 invasive CSCCs with metastasis, and 30 cases with normal cervical squamous epithelia. Western blot analysis was used to detect the expression of RAGE in CSCC. Results： The positive expression rates of RAGE were 48.0% in CSCC （ 72/150 ） and 13.3% in normal squamous epithelial tissues of the cervix （ 20/150 ）, indicating significant differences between the two conditions. The overexpression of RAGE was correlated with tumor progression, stage, invasion, and metastasis of CSCC （ P 〈 0.01 ）, but it had no relation with the degree of differentiation and tumor size （ P 〉 0.05 ）. Conclusion： Overexpression of RAGE in CSCC, especially in cervical squamous epithelial tissues with tumor metastasis, can be used as one of the parameters for determining tumor invasion, metastasis, and prognosis.

关 键 词：宫颈鳞癌 晚期糖基化终产物受体 肿瘤浸润 

分 类 号：R737.33[医药卫生—肿瘤]