番茄红素-β-环化酶(LYC-b)反义基因在番茄转基因后代的表达及其遗传稳定性  

Expression and Genetic Stability of Lycopene-β-cyclase (LYC-b) Antisense Gene in the Progeny of Transgenic Tomato

作　　者：吴江敏[1] 孙亚东[1] 梁燕[1] 徐加新[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,杨凌712100

出　　处：《农业生物技术学报》2011年第5期801-807,共7页Journal of Agricultural Biotechnology

基　　金：科技部农业科技成果转化基金项目(No.2009GB2G000362);陕西省13115重大科技专项(No.2009ZDKG-14)共同资助

摘　　要：为了进一步明确番茄红素-β-环化酶（LYC-b）反义基因在番茄转基因后代的遗传表现,在已成功获得转LYC-b反义基因植株的基础上,本实验通过对反义LYC-b基因的检测、果实番茄红素含量与可溶性固形物含量的测定以及形态特征和生物学特性田间观察,进一步对2个转LYC-b反义基因的番茄（Lycopersicum esculentum）品种的T1和T2群体进行遗传及作用稳定性研究。结果表明,从T1阳性植株获得的T2植株均检测出了LYC-b反义基因的存在和表达,表明目的基因在世代之间能够稳定遗传。LYC-b反义基因对果实番茄红素含量的影响在不同品种、不同世代以及同一世代不同单株之间均有差异。T1转基因植株番茄红素含量比对照提高24%-72%;T2转基因植株番茄红素含量平均值比对照提高24.5%-105.3%。两个品种不同世代转基因植株的每序花数和每序果数均低于阴性对照;转基因番茄果实的可溶性固形物含量在不同世代以及不同单株之间均有不同层度的变化;T2有一株生长习性由无限突变为有限生长型。研究结果为获得具有稳定表型的能够在生产中推广应用的转基因品系提供了基础资料。In order to clarify the genetic presentation of the Lycopene-β-cyclase（LYC-b） antisense gene in the progeny of transgenic tomato, the genetic stability of transgenic LYC-b antisense gene of T1 and T2 progenies of tomato（Lycopersicum esculentum） 2 varieties were studied, based on the previous getting transgenic LYC-b antisense gene plant, through detection of transgenicLYC-b antisense gene, measurement of fruit lycopene and soluble solid contents, and morphological and biological characteristics. The results showed that LYC-b antisense gene was exsisted and expressed in T2 plants, which was from positive T1 plant. This means target gene can be stably inherited from one generation to another. There were some differences between cultivars, generations, and plants of the same generation in the effect of LYC-b antisense gene on fruit lycopene content. The fruit lycopene content of T1 transgenetic plants increased by 24%~72% compare to the control while T1 transgenetic plantsincreased by 24.5%~105.3%. In addition, the flower number per infloresence was less thanthat of the negative control; the soluble solids content of transgenic tomato plants was different between generations and plants; one of T2 generation plants mutated from unlimited growth type to limited one. The results provides some basic data for extending the stable phenotype of transgenic tomato strains in production.

关 键 词：番茄 LYC-b反义基因 番茄红素 

分 类 号：S511[农业科学—作物学]