肿瘤浸润性树突状细胞在子宫内膜癌中的凋亡及其Fas、FasL表达意义  

Apoptosis and expression of Fas/FasL in tumor infiltrating dendritic cells in human endometrioid adenocarcinoma

作　　者：贾建军[1] 王自能[1] 刘革修[2] 王志新[1] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632

出　　处：《南方医科大学学报》2011年第10期1693-1696,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30670902);广东省医学科学技术研究基金(A2009361)~~

摘　　要：目的探讨子宫内膜癌浸润性树突状细胞(TIDC)凋亡及其凋亡相关蛋白Fas/FasL的表达意义。方法利用S-100单克隆抗体和DNA缺口末端标记(TUNEL法)相结合,分析45例子宫内膜癌组织中TIDC凋亡状况;通过免疫组织化学双重染色方法检测TIDC表面Fas/FasL分子表达。结果子宫内膜癌组织中TIDC凋亡发生率显著高于子宫内膜组织中树突状细胞凋亡发生率[(13.02±0.64)%vs(6.82±0.53)%,P<0.05];子宫内膜癌组织TIDC膜表面Fas表达显著低于正常子宫内膜组织(7.88±1.05 vs 19.25±3.03,P<0.05),子宫内膜癌组织TIDC膜表面FasL表达显著高于正常子宫内膜组织(12.95±2.25 vs 7.51±1.14,P<0.05)。结论子宫内膜癌组织中TIDC凋亡增加及Fas、FasL在TIDC上表达失衡,可能导致子宫内膜癌免疫逃逸发生。Objective To investigate apoptosis of tumor infiltrating dendritic cells（TIDC） and their expression of Fas/FasL（CD95/CD95L） in human endometrioid adenocarcinoma.Methods The apoptotic rate of TIDC was measured in 45 cases of endometrioid adenocarcinoma and 20 cases of normal endometrium tissues（control） by double-label immunohistochemistry using the monoclonal antibody S-100 protein and TUNEL technique.The expressions of Fas and FasL in TIDCs were detected using double-label immunohistochemistry and imaging analysis.Results The apoptotic rate of TIDCs in endometrioid adenocarcinoma were significantly higher than that in normal endormetrium [（13.02±0.64）% vs（6.82±0.53）%,P0.05].The expression levels of Fas in the TIDCs were significantly lower,whereas FasL expression significantly higher in endometrioid adenocarcinoma than in normal endormetrium（7.88±1.05 vs 19.25±3.03,P0.05;12.95±2.25 vs 7.51±1.14,P0.05）.Conclusion Increased apoptosis of the TIDCs and abnormal expression of Fas/FasL in TIDCs in endometrioid adenocarcinoma may lead to tumor immune escape.

关 键 词：子宫内膜癌 肿瘤浸润性树突状细胞 凋亡 FAS FASL 

分 类 号：R737.33[医药卫生—肿瘤]