Tiam1蛋白在肺癌组织中表达的定量分析及临床意义  

Quantitative analysis of Tiam1 expression in lung cancer and its clinical significance

作　　者：李玉梅[1,2] 齐文娟[1,2] 申洪[1,2] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515 [2]南方医科大学基础医学院病理学系,广东广州510515

出　　处：《南方医科大学学报》2011年第10期1774-1777,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2010B060300001)

摘　　要：目的探讨T淋巴瘤侵袭和转移诱导因子1(Tiam1)的表达与肺癌侵袭转移、TNM分期及组织学类型的关系。方法用免疫组织化学技术检测116例肺癌组织及20例淋巴结转移灶中Tiam1的表达;用Nikon显微镜通过Canon数码像机和Zoom Browser Ex采图软件随机截取样品阳性部位图像,用Imagepro Plus图像分析软件定量测试各例样品中Tiam1蛋白表达的阳性单位(PU值)。结果有淋巴结转移组Tiam1的PU值大于无淋巴结转移组Tiam1的PU值(P=0.039);TNM分期TNMⅡ-Ⅳ期Tiam1的PU值大于TNMⅠ期Tiam1的PU值(P=0.025);淋巴结转移灶中Tiam1的PU值大于原发灶中Tiam1的PU值(P=0.000);肺鳞状细胞癌Tiam1的PU值分别小于肺腺癌Tiam1的PU值(P=0.002)、肺大细胞癌Tiam1的PU值(P=0.027)及肺小细胞癌Tiam1的PU值(P=0.024)。肺癌中Tiam1的表达强度与患者性别、年龄、吸烟史、肺尘埃情况、肿瘤大体类型和分化程度无关。结论 Tiam1可能也是肺癌的一个促侵袭转移因子,其表达强度与肺癌的淋巴结转移、TNM分期和组织学类型密切相关。Objective To investigate the relationship between the expression of T lymphoma invasion and metastasis inducing factor 1（Tiam1） and the progression,metastasis,TNM stage,and histological types of lung carcinoma.Methods Immunohistochemistry was performed to detect the expression of Tiam1 in 116 lung carcinoma specimens.The expression intensity（measured in positive unit,PU） of Tiam1 in these tissues was assessed quantitatively using Imagepro Plus image analysis software.Results The PU of Tiam1 was significantly greater in primary lung carcinomas with lymph node metastases than in those without metastases（t=-2.089,P=0.039）.Lung cancers of TNM stage II-IV had stronger expression than those of stage I（t=-2.272,P=0.025）.The PU of Tiam1 differed significantly between different histological types of lung cancer,and squamouscell cell carcinoma had a lower PU than adenocarcinoma,large cell carcinoma and small cell carcinoma（P0.05）.The intensity of Tiam1 expression was not associated with the patients＇ gender,age,general types,smoking history,pneumoconiosis or differentiation of lung carcinoma.Conclusion These results strongly suggest that Tiam1 is an invasion and metastasis inducing factor of lung carcinoma.The overexpression of Tiam1 is closely associated with lymph node metastases,TNM stage and histological types of lung carcinoma.

关 键 词：肺肿瘤 T淋巴瘤侵袭和转移诱导因子1 肿瘤转移 TNM分期 

分 类 号：R734.2[医药卫生—肿瘤]