肠道病毒71型VP1蛋白二级结构及B细胞表位的预测  

Prediction on secondary structure and B cell epitope of enterovirus type 71 for VP1 gene

作　　者：王锦[1] 许国章[2] 倪红霞[2] 

机构地区：[1]宁波大学医学院,宁波315211 [2]宁波市疾病预防控制中心,宁波315010

出　　处：《中国人兽共患病学报》2011年第10期905-908,共4页Chinese Journal of Zoonoses

基　　金：浙江省卫生厅医药卫生科学研究基金资助项目(2009A190);宁波市科技局重大科技专项资助项目(2009C50008)

摘　　要：目的预测C4基因亚型肠道病毒71型的VP1蛋白二级结构及B细胞表位,为EV71疫苗研制提供理论基础。方法本研究将宁波地区EV71分离株与国内外EV71代表株进行同源性分析和并构建亲缘进化树。以VP1基因序列为材料,应用EX-PASY服务器上的GOR4、SOPMA两种方法分析预测蛋白质二级结构,并结合蛋白质的亲水性、柔韧性、表面可能性等指标综合评价VP1蛋白的B细胞抗原表位。结果同源性分析和亲缘进化分析得出,2010年宁波EV71分离株属于C4a基因亚型,与基因库检索到的C4a基因亚型代表株核苷酸和氨基酸同源性较高,分别为93.6%～99.3%和98.3%～100%。VP1蛋白二级结构以无规则卷曲和β-片层为主,有多处抗原指数较高的区段,结合亲水性、柔韧性、表面可能性等指标,综合预测VP1蛋白的B细胞抗原表位在第39～40、159～167、212～220、287～290位氨基酸残基的可能性较大。结论 EV71VP1蛋白多个区段具有抗原潜力通过生物信息学方法预测VP1蛋白二级结构及B细胞表位为EV71疫苗研制提供理论基础。The purpose of this study was to predict the secondary structure and B cell epitopes of enterovirus type 71 of VP1 Gene of C4 subgenotype,so as to provide theory evidence for the study of vaccine preparation for EV71.EV71 strains were isolated from hand,foot and mouth disease in Ningbo in 2010.Homology and phylogenetic analysis based on VP1 with others EV71 have published in GenBank were conducted.The secondary structure of EV71 based on VP1 was analyzed using the GOR4 and SOPMA,and the B cell epitope of EV71 was predicted by combining the hydrophilicity、flexility and surface probability.The Ningbo strain shared the highest homology of nucleotide and amino acid with the type C4a were 93.6%-99.3% and 98.3%-100%,phylogenetic analysis revealed that Ningbo strains clusterd within the C4a evolution branch of C4 subgenotype.The secondary structure of EV71 VP1 showed random coils and β-sheet mainly with a number of antigen index higher section.The B cell epitopes was probably located at the regions of 39-40,159-167,212-220,and 287-290 of VP1 Gene.The secondary structure and B cell epitopes of EV71 VP1 could be predicted by bioinformatics methods,which providing theory evidence for the study of vaccine preparation for EV71.

关 键 词：肠道病毒71型 VP1 蛋白质二级结构 B细胞表位 

分 类 号：R373.2[医药卫生—病原生物学]