维甲酸对人食管癌细胞核基质蛋白表达的影响  

The effect of retinoic acid on expression of nuclear matrix protein in human esophageal cancer cells

作　　者：杨海波[1] 陈兰英[1] 孙婕[1] 谢朝晖[1] 

机构地区：[1]河南城建学院生物工程系,河南平顶山467036

出　　处：《癌变．畸变．突变》2011年第5期377-379,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30470877);河南省重点科技攻关项目(092102310006);河南省教育厅基金项目(2009B180001)

摘　　要：目的:探讨维甲酸对人食管癌EC9706细胞核基质蛋白表达的影响,了解食管癌变与核基质蛋白的关系。方法:人食管癌EC9706细胞采用维甲酸诱导分化处理48h后,进行核基质蛋白提取,采用双向电泳技术检测维甲酸处理前后核基质蛋白表达的变化。结果:维甲酸处理前后的EC9706细胞中多种核基质蛋白的表达发生了变化,其中处理后表达下调的有4个核基质蛋白,表达上调的11个,新出现的有3个蛋白质点。结论:维甲酸对体外培养的人食管癌EC9706细胞核基质蛋白的表达种类及水平有显著影响,核基质蛋白表达的变化可能与食管的癌变有关。OBJECTIVE：To study the effect of retinoic acid on expression of nuclear matrix protein in human esophageal cancer cell EC9706,in order to further understanding of the relationship between esophageal carcinogenesis and nuclear matrix proteins.METHODS：Through cell culture and induction of differentiation treatment for 48 h, nuclear matrix protein selective extraction,two dimensional gel electrophoresis analysis to detect the expression of nuclear matrix protein in human esophageal carcinoma EC9706 before and after retinoic acid treatment.RESULTS：The expression of nuclear matrix protein showed great changes after treatment with retinoic acid in EC9706 cells.There were 18 marked changes during apoptosis induced by retinoic acid,with 11 up-regulated proteins,the 4 down-regulated proteins,and 3 new spots.CONCLUSION：This study suggested that the types and expression levels of nuclear matrix protein could change significantly after treatment by retinoic acid in human esophageal cancer EC9706 cells.

关 键 词：食管癌 EC9706细胞 维甲酸 核基质蛋白 

分 类 号：Q247[生物学—细胞生物学] Q28