重组人乳铁蛋白的遗传毒性研究  

Genetic toxicity of recombinant human lactoferrin

作　　者：刘珊[1] 冯晓莲[1] 李晨汐[1] 耿桂英[1] 刘海波[1] 支媛[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《癌变．畸变．突变》2011年第5期384-387,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家高技术研究发展计划(863计划)项目(2007AA100506);转基因生物新品种培育科技重大专项(2008ZX08011-005)

摘　　要：目的:评价重组人乳铁蛋白的遗传毒性。方法:以重组人乳铁蛋白为受试物,按每皿62、185、556、1667、5000μg5个剂量进行Ames试验,按1.88、3.75和7.50g/kg进行小鼠骨髓嗜多染红细胞(PCE)微核试验和精子畸形试验,并设立对照组。结果:重组人乳铁蛋白各剂量组回变菌落数均未超过溶剂对照菌落数的2倍,亦无剂量-反应关系;各剂量组PCE百分比均未少于阴性对照组的20%,微核发生率与阴性对照组比较差异均无统计学意义(P均>0.05);各剂量组小鼠精子畸形发生率均显著低于阴性对照组(P<0.05)。结论:在本实验条件下,重组人乳铁蛋白未见遗传毒性。OBJECTIVE：To evaluate genetic toxicity of recombinant human lactoferrin（rhLf） expressed in milk from genetically modified cow.METHODS：In Ames test,TA97,TA98,TA100,and TA102 strains were treated with 62,185,556,1 667 and 5 000μg recombinant human lactoferrin（rhLf） per plate.In mice bone marrow cell micronucleus test and mice sperm abnormality test,1.88,3.75 and 7.50 g/kg rhLf groups and control groups were set.RESULTS：Back mutation colonies of rhLf groups did not exceed more than twice that of the control in Ames test and there was no dose-response relationship.In mice bone marrow cell micronucleus test,no significant difference was found in PCE/RBC and micronucleus rate between rhLf-treated groups and negative control group.In mice sperm abnormality test,sperm abnormality rate of rhLf-treated groups was lower than that of negative control.CONCLUSION：No genetic toxicity was observed in recombinant human lactoferrin in this study.

关 键 词：遗传毒性 重组蛋白 人乳铁蛋白 

分 类 号：R114[医药卫生—卫生毒理学] R15[医药卫生—公共卫生与预防医学]