青藤碱对肺癌A549细胞增殖的抑制作用  

Sinomenine inhibits proliferation of A549 human lung cancer cells

作　　者：杨国才[1] 雨山[2] 徐广全[2] 

机构地区：[1]浙江省舟山医院总部胸外科,舟山316000 [2]哈尔滨医科大学附属第二临床医学院胸外科,哈尔滨150086

出　　处：《中国中西医结合外科杂志》2011年第5期482-485,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的:研究青藤碱对肺癌A549细胞增殖的影响及其机制。方法:以不同浓度青藤碱分别处理体外培养的A549细胞,采用MTT法检测24h、48h、72h后A549细胞的增殖状态,流式细胞仪测定细胞凋亡;细胞免疫组化检测Cox-2蛋白表达量。结果:以0.1、0.25、0.5、1.0、2.0、2.5、5.0、10.0mmol/L青藤碱作用于A549细胞后,细胞增殖明显受到抑制,呈时间和剂量依赖性。青藤碱处理组细胞早期凋亡率较对照组升高,呈时间和剂量依赖性;Cox-2蛋白表达明显抑制。结论:青藤碱在体外能有效抑制肺癌细胞增殖。Objective To study the anti-proliferation of Sinomenine on A549 human lung cancer cells and its mechanism.Methods A549 cells were treated with various concentration of Sinomenine for 24、48 and 72 h.Cell proliferation was measured by MTT assay,and cell apoptosis was examined by flow cytometry.Cox-2 protein was tested by immunohistochemistry test.Results Sinomenine （0.1、0.25、0.5、1.0、2.0、2.5、5.0、10.0 mmol/L） could significantly induce anti-proliferation effect of A549 cells in a dose-and time-dependent manner.The early apoptosis rates after Sinomenine treated were higher than the control group,and the apoptosis rates were positively correlated with the concentration and time of Sinomenine treatment.In additional,the cox-2 protein expression was significantly inhibited.Conclusion Sinomenine could effectively inhibit proliferation of A549 human lung cancer cells in vitro.

关 键 词：青藤碱 肺癌 A549 细胞凋亡 环氧化酶2 

分 类 号：R734.2[医药卫生—肿瘤]