高血压病患者大网膜小动脉重塑的观测及相关蛋白表达探讨  

Omental arteriole remodeling and related protein expression in hypertensive patients

作　　者：孙敬昌[1] 齐冬梅[2] 李运伦[3] 

机构地区：[1]山东中医药大学基础医学院细胞生物学实验室,山东济南250355 [2]山东中医药大学科技处,山东济南250355 [3]山东中医药大学附属医院心内科,山东济南250011

出　　处：《中国病理生理杂志》2011年第10期1863-1867,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30300466);泰山学者建设工程专项经费资助项目(No.鲁办发[2004]28号)

摘　　要：目的:研究高血压病患者大网膜小动脉病理损害、重塑及相关蛋白的表达。方法:检测12例非高血压病对照者和12例高血压病患者血液流变学及大网膜小动脉形态学的变化,采用免疫组织化学方法检测大网膜小动脉Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达,用Masson染色法观察大网膜小动脉壁胶原含量变化。结果:高血压病患者血液流变学异常,大网膜小动脉胶原沉积增多,中膜厚度/管腔直径比值增大,动脉壁Bcl-2、Bax、c-Myc、c-Fos、MMP-9和TIMP-2蛋白表达增强。结论:高血压病患者大网膜小动脉存在病理损害和重塑,其机制与血液动力学(高血压)和血液流变学异常,动脉壁Bcl-2和Bax、MMP-9和TIMP-2表达失衡及c-Myc和c-Fos过度表达有关。AIM：To study the pathological changes,adverse remodeling and related protein expression of omental arteriole in hypertensive patients.METHODS： Twelve non-hypertensive persons and 12 hypertensive patients were selected,and the hemorheology and morphological changes of their omental arterioles were detected.The protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 in the arterioles of omental tissues was determined by the method of immunohistochemistry.The changes of collagen content in omental arterioles were observed by Masson staining.RESULTS： Compared with the non-hypertensive persons,the hemorheological abnormalities,increased omental arteriole collagen deposition,widened mid layer thickness/lumenal diameter ratio and enhanced protein expression of Bcl-2,Bax,c-Myc,c-Fos,MMP-9 and TIMP-2 were observed in hypertensive patients.CONCLUSION： The pathological damage and adverse remodeling exist in omental arterioles of hypertensive patients.The mechanism may be related with the hymodynamic of hypertension,hemorheological abnormalities,imbalanced expression of Bcl-2,Bax,MMP-9 and TIMP-2,and concerned with the over-expression of c-Myc and c-Fos in the arteriolar wall.

关 键 词：高血压病 大网膜小动脉 血管重塑 细胞凋亡 基因蛋白表达 

分 类 号：R363[医药卫生—病理学]