白血病Reh、HL-60、K562细胞系RUNX3基因P2启动子甲基化状态与RUNX3基因表达  

Methylation status of RUNX3gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh,HL-60 and K562

作　　者：李大彩[1] 吴明彩[1] 张根葆[2] 毕富勇[1] 

机构地区：[1]皖南医学院肿瘤生化研究室,安徽芜湖241002 [2]皖南医学院病理生理学教研室,安徽芜湖241002

出　　处：《中国病理生理杂志》2011年第10期2017-2020,共4页Chinese Journal of Pathophysiology

基　　金：安徽省高校自然科学基金资助项目(No.KJ2010B249)

摘　　要：目的:研究人白血病Reh、HL-60与K562细胞系RUNX3基因P2启动子甲基化状态及RUNX3基因的表达,分析P2启动子甲基化与基因表达之间的关系。方法:运用甲基化特异性PCR(MSP)和RT-PCR检测Reh、HL-60与K562细胞系RUNX3基因P2启动子的甲基化状态及RUNX3基因的表达。结果:Reh及K562细胞系RUNX3基因P2启动子甲基化特异性扩增呈阳性,非甲基化特异性扩增呈阴性,RT-PCR扩增呈阴性;而HL-60细胞系相反,甲基化特异性扩增呈阴性,而非甲基化特异性扩增呈阳性,RT-PCR扩增阳性。结论:Reh及K562细胞中存在RUNX3基因P2启动子的甲基化,且在不同类型白血病细胞系中的甲基化状态不同。AIM：To investigate the relationship between the methylation status of RUNX3 gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh,HL-60 and K562.METHODS： Methylation status of RUNX3 gene P2 promoter was detected by methylation-specific polymerase chain reaction（MSP） and the mRNA expression of RUNX3 was determined by RT-PCR in leukemic cell lines Reh,HL-60 and K562.RESULTS： Methylation-specific PCR products were detected in Reh cells and K562 cells,while those in HL-60 cells were negative.Unmethylation-specific PCR products were only detected in HL-60 cells.RT-PCR amplification of RUNX3 was negative in Reh cells and K562 cells,but that was positive in HL-60 cells.CONCLUSION： Methylation in P2 promoter of RUNX3 gene exists in human leukemic Reh cells and K562 cells.There is different methylation status in different types of leukemic cell lines.

关 键 词：白血病 基因 RUNX3 甲基化特异性PCR 

分 类 号：R361.3[医药卫生—病理学]