红景天苷促进培养乳鼠心肌细胞肌质网钙离子的释放  

Salidroside increases release of intracellular free Ca^(2+) from sarcoplasmic reticulum in cultured rat cardiomyocytes

作　　者：庞勇军[1,2] 孙莉[3] 谢文娟[4] 莫书荣[2] 

机构地区：[1]桂林医学院生理学教研室,广西桂林541004 [2]广西医科大学生理学教研室,广西南宁530021 [3]桂林医学院组织学与胚胎学教研室,广西桂林541004 [4]桂林医学院生药学教研室,广西桂林541004

出　　处：《中国病理生理杂志》2011年第10期2021-2023,2026,共4页Chinese Journal of Pathophysiology

基　　金：广西自然科学基金资助项目(No.0728140)

摘　　要：目的:观察红景天苷对乳鼠心肌细胞胞浆Ca2+浓度的影响并分析其可能的作用机制。方法:应用荧光指示剂Fluo-3/AM负载培养大鼠乳鼠的心肌细胞,用激光共聚焦显微镜动态观察胞内游离钙荧光信号强度的变化,检测不同浓度红景天苷对培养心肌细胞胞内游离钙离子浓度([Ca2+]i)的影响。结果:红景天苷浓度为15 mg/L、30 mg/L和60 mg/L时,细胞内的平均[Ca2+]i升高,峰值分别为574.08±4.65、591.86±3.64和618.66±4.27(均P<0.01);有剂量依赖性而无时间依赖性。用维拉帕米阻断细胞膜外钙内流时,红景天苷同样引起细胞内[Ca2+]i升高,峰值由357.74±3.13、387.17±2.37和391.43±1.34分别上升到480.86±3.98、496.70±3.08和522.18±3.19(均P<0.01)。结论:红景天苷能升高乳鼠心肌细胞中[Ca2+]i,其机制可能与其促进肌浆网钙离子释放有关。AIM：To observe the effects of salidroside on intracellular free Ca2＋ concentration in cultured rat cardiomyocytes.METHODS： Primarily cultured cardiomyocytes of neonatal rats were divided into control group,different concentrations of salidroside groups and verapamil pretreatment＋different concentrations of salidroside groups.The fluorescent intensity of intercellular free calcium concentration（i） in cultured cardiomyocytes of newborn rats loaded with fluo-3/AM（5 μmol/L） was measured by laser scanning confocal microscopy.RESULTS： Salidroside at concentrations of 15 mg/L,30 mg/L and 60 mg/L elevated i in cultured rat cardiomyocytes with the peak values of 574.08±4.65,591.86±3.64 and 618.66±4.27,respectively（all P〈0.01）,indicating that the effect of salidroside on the level of i was dose-dependent.In the presence of verapamil in D-Hanks solution,salidroside also elevated the fluorescent intensity of i in cardiomyocytes from 357.74±3.13,387.17±2.37 and 391.43±1.34 to 480.86±3.98,496.70±3.08 and 522.18±3.19,respectively（all P〈0.01）.CONCLUSION： Salidroside increases the release of i from sarcoplasmic reticulum in cultured rat cardiomyocytes.

关 键 词：红景天苷 钙 心肌细胞 心肌收缩 

分 类 号：R363[医药卫生—病理学]