微管相关蛋白2、巢蛋白与人胚胎食管肌层发育的关系  

Relationship between MAP-2,nestin and development of muscular layer of human embryonic esophagus

作　　者：张泳[1] 刘学红[2] 

机构地区：[1]绍兴文理学院医学院医学实验中心,浙江绍兴312000 [2]绍兴文理学院医学院组织学与胚胎学教研室,浙江绍兴312000

出　　处：《中国病理生理杂志》2011年第10期2024-2026,共3页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅科研计划资助项目(No.Y201018535);绍兴文理学院重点项目(No.2010LG1011)

摘　　要：目的:探讨微管相关蛋白2(MAP-2)和巢蛋白(nestin)与人胚胎食管肌层发育关系。方法:应用免疫组织化学PV法检测第2、3、4月龄段人胚胎食管组织内MAP-2和nestin的表达分布规律。结果:第2、3、4月龄段,MAP-2在食管肌层的肌细胞呈阴性表达,肌间神经丛内神经细胞处呈阳性表达;nestin在第2-3月龄食管肌层的肌细胞处呈阳性表达,到第4月龄转阴性表达;而在食管肌间神经丛内大部分神经细胞,第2-4月龄段呈弱阳性或阴性表达。结论:MAP-2和nestin可能参与调节人胚胎食管肌层的形成过程。AIM：To explore the relationship between microtubule-associated protein 2（MAP-2）,nestin and development of esophageal muscular layer in human embryos.METHODS： At the second to fourth month of gestation,the protein expression of MAP-2 and nestin was observed using the PV method of immunohistochemistry in the human embryonic esophageal tissues.RESULTS： At the second month,the third month and the fourth month of gestation,MAP-2 positive expression in the neural cells of the intermuscular nerve plexus,and negative expression in the muscle cells of human embryonic developing esophageal muscle were observed.Nestin positive expression was found in the muscle cells at the second month to the third month of gestation.However,no nestin expression in the muscle cells of the esophageal muscle at the fourth month of gestation was observed.At the second month to the fourth month of gestation,nestin showed negative or weak positive expression in the neural cells of the intermuscular nerve plexus of human embryonic esophageal tissues.CONCLUSION： MAP-2 and nestin might regulate the formation of esophageal muscular layer in human embryos.

关 键 词：食管 微管相关蛋白2 巢蛋白 人胚胎 

分 类 号：R[医药卫生]